A sensitive LC-MS/MS method for quantifying clofarabine triphosphate concentrations in human peripheral blood mononuclear cells
文献类型：期刊论文
| 作者 | Tu, Xiaowei; Lu, Youming; Zhong, Dafang ; Zhang, Yifan; Chen, Xiaoyan
|
| 刊名 | JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
 |
| 出版日期 | 2014-08-01 |
| 卷号 | 964页码:202-207 |
| 关键词 | Clofarabine Clofarabine triphosphate Liquid chromatography-tandem mass spectrometry Peripheral blood mononuclear cells |
| ISSN号 | 1570-0232 |
| DOI | 10.1016/j.jchromb.2014.01.021 |
| 文献子类 | Article |
| 英文摘要 | Clofarabine triphosphate is an intracellular active metabolite of clofarabine. In the present study, we developed and validated a rapid, sensitive, and selective liquid chromatography-tandem mass spectrometry method (LC-MS/MS) for quantifying clofarabine triphosphate concentrations in human peripheral blood mononuclear cells (PBMCs). PBMCs were isolated from blood using the Ficoll gradient centrifugation method. Chromatographic separation was performed on a CN column using an isocratic mobile phase comprising acetonitrile/5 mM ammonium acetate with 0.001% ammonium hydroxide (20/80, v/v) at a flow rate of 0.60 mL/min. Detection was carried out by MS/MS in the multiple reaction monitoring mode using a negative electrospray ionization interface. The method was validated in concentration ranges of 1.25-100 ng/10(7) cells with acceptable accuracy and precision using 50 mu L of cell extract. Clofarabine triphosphate was stable in a series of stability studies with bench-top, auto-sampler, and repeated freeze-thaw cycles. The validated method was successfully used to measure the concentrations of clofarabine triphosphate in PBMCs from cancer patients treated with clofarabine (C) 2014 Elsevier B.V. All rights reserved. |
| WOS关键词 | PERFORMANCE LIQUID-CHROMATOGRAPHY ; NUCLEOSIDE TRANSPORTERS ; LEUKEMIA ; QUANTITATION ; CANCER ; ANALOG ; DRUGS |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000338977000021 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/276975]  |
| 专题 | 上海药物代谢研究中心 |
| 通讯作者 | Chen, Xiaoyan |
| 作者单位 | Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Tu, Xiaowei,Lu, Youming,Zhong, Dafang,et al. A sensitive LC-MS/MS method for quantifying clofarabine triphosphate concentrations in human peripheral blood mononuclear cells[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2014,964:202-207. |
| APA | Tu, Xiaowei,Lu, Youming,Zhong, Dafang,Zhang, Yifan,&Chen, Xiaoyan.(2014).A sensitive LC-MS/MS method for quantifying clofarabine triphosphate concentrations in human peripheral blood mononuclear cells.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,964,202-207. |
| MLA | Tu, Xiaowei,et al."A sensitive LC-MS/MS method for quantifying clofarabine triphosphate concentrations in human peripheral blood mononuclear cells".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 964(2014):202-207. |
入库方式： OAI收割
来源：上海药物研究所
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