A chiral high-performance liquid chromatography-tandem mass spectrometry method for the stereospecific determination of morinidazole in human plasma
文献类型：期刊论文
| 作者 | Zhong, Kan2; Gao, Zhiwei2 ; Li, Qin1; Zhong, Dafang2; Chen, Xiaoyan2
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| 刊名 | JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
 |
| 出版日期 | 2014-06-15 |
| 卷号 | 961页码:49-55 |
| 关键词 | Morinidazole enantiomer Liquid chromatography-tandem mass spectrometry Stereoselective pharmacokinetics 5-Nitroimidazole antimicrobial drug |
| ISSN号 | 1570-0232 |
| DOI | 10.1016/j.jchromb.2014.04.049 |
| 文献子类 | Article |
| 英文摘要 | Morinidazole is a novel 5-nitroimidazole derivative used for the treatment of amoebiasis, trichomoniasis, and anaerobic bacterial infections. Morinidazole possesses a chiral carbon and is clinically administered as a racemate. In the present study, an enantioselective and sensitive liquid chromatography tandem mass spectrometry method of determining morinidazole enantiomers in human plasma was developed and validated to characterize the stereoselective pharmacokinetics. Plasma samples were processed by liquid liquid extraction using tert-butyl methyl ether. Chiral separation was optimized within 8.5 min on a cellulose column using an isocratic mobile phase of methanol/water (80:20, v/v). Detection was using mass spectrometry in multiple reaction monitoring mode, using the transitions of m/z 271 -> 144 for morinidazole enantiomers, and m/z 275 -> 148 for d(4)-morinidazole (internal standard). The calibration curves were linear over 5.00-6000 ng/mL for each enantiomer. The lower limit of quantification for each enantiomer was established at 5.00 ng/mL. Intra- and inter-day precisions were less than 6.4% for each enantiomer in terms of relative standard deviation, and accuracies were between -2.5% and 6.4% in terms of relative error for each enantiomer. No chiral inversion was observed during sample storage, preparation procedure and analysis. Major glucuronide and sulfate conjugates were not observed to interfere with the determination of morinidazole enantiomers. The method was applied to study the stereoselective pharmacokinetics of morinidazole in humans. Moderate stereoselectivity was observed in healthy subjects and patients with severe renal impairment. (C) 2014 Elsevier B.V. All rights reserved. |
| WOS关键词 | TRICHOMONAS-VAGINALIS ; N+-GLUCURONIDES ; METRONIDAZOLE |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000337863300008 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/277021]  |
| 专题 | 上海药物代谢研究中心 |
| 通讯作者 | Chen, Xiaoyan |
| 作者单位 | 1.Jiangsu Hansoh Pharmaceut Co Ltd, Lianyungang 222069, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Zhong, Kan,Gao, Zhiwei,Li, Qin,et al. A chiral high-performance liquid chromatography-tandem mass spectrometry method for the stereospecific determination of morinidazole in human plasma[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2014,961:49-55. |
| APA | Zhong, Kan,Gao, Zhiwei,Li, Qin,Zhong, Dafang,&Chen, Xiaoyan.(2014).A chiral high-performance liquid chromatography-tandem mass spectrometry method for the stereospecific determination of morinidazole in human plasma.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,961,49-55. |
| MLA | Zhong, Kan,et al."A chiral high-performance liquid chromatography-tandem mass spectrometry method for the stereospecific determination of morinidazole in human plasma".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 961(2014):49-55. |
入库方式： OAI收割
来源：上海药物研究所
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