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Enantioselective determination of ornidazole in human plasma by liquid chromatography-tandem mass spectrometry on a Chiral-AGP column

文献类型：期刊论文

作者Du, Jiangbo1; Ma, Zhiyu1; Zhang, Yifan1; Wang, Ting2; Chen, Xiaoyan1; Zhong, Dafang1
刊名JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
出版日期2013-12
卷号86页码:182-188
关键词Ornidazole Enantiomers Enantioselective liquid chromatography-tandem mass spectrometry Stereoselective pharmacokinetics
ISSN号0731-7085
DOI10.1016/j.jpba.2013.07.048
文献子类Article
英文摘要A rapid, sensitive, and enantioselective method was developed and validated for determination of ornidazole enantiomers in human plasma by liquid chromatography-tandem mass spectrometry. Ornidazole enantiomers were extracted from 100 mu l of plasma using ethyl acetate. Baseline chiral separation (R-s = 2.0) was obtained within 7.5 min on a Chiral-AGP column (150 mm x 4.0 mm, 5 mu m) using an isocratic mobile phase of 10 mM ammonium acetate/acetic acid (100/0.01, v/v). Stable isotopically labeled R-(+)-d(5)-ornidazole and S-(-)-d(5)-ornidazole were synthesized as internal standards. Acquisition of mass spectrometric data was performed in multiple reaction monitoring mode via positive electrospray ionization, using the transitions of m/z 220 -> 128 for ornidazole enantiomers, and m/z 225 -> 128 for d(5)-ornidazole enantiomers. The method was linear in the concentration range of 0.030-10.0 mu g/ml for each enantiomer. The lower limit of quantification for each enantiomer was 0.030 mu g/ml. The relative standard deviation values of intra- and inter-day precision were 1.8-6.2% and 1.5-10.2% for R-(+)-ornidazole and S-(-)-ornidazole, respectively. The relative error values of accuracy ranged from -4.5% to 1.2% for R-(+)-ornidazole and from -5.4% to -0.8% for S-(-)-ornidazole. The validated method was successfully applied to a stereoselective pharmacokinetic study of ornidazole after oral administration of 1000 mg racemic ornidazole. (C) 2013 Elsevier B.V. All rights reserved.
WOS关键词ANAEROBIC-BACTERIA ; STEREOSELECTIVE DETERMINATION ; RAT PLASMA ; LC-MS/MS ; ENANTIOMERS ; METRONIDAZOLE ; QUANTIFICATION ; QUANTITATION ; MECHANISM
WOS研究方向Chemistry ; Pharmacology & Pharmacy
语种英语
WOS记录号WOS:000325671200021
出版者ELSEVIER SCIENCE BV
源URL[http://119.78.100.183/handle/2S10ELR8/277369]  
专题上海药物代谢研究中心
通讯作者Zhong, Dafang
作者单位1.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China;
2.Lanzhou Univ, Affiliated Hosp 1, Lanzhou 730000, Peoples R China
推荐引用方式
GB/T 7714		 Du, Jiangbo,Ma, Zhiyu,Zhang, Yifan,et al. Enantioselective determination of ornidazole in human plasma by liquid chromatography-tandem mass spectrometry on a Chiral-AGP column[J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,2013,86:182-188.
APA Du, Jiangbo,Ma, Zhiyu,Zhang, Yifan,Wang, Ting,Chen, Xiaoyan,&Zhong, Dafang.(2013).Enantioselective determination of ornidazole in human plasma by liquid chromatography-tandem mass spectrometry on a Chiral-AGP column.JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,86,182-188.
MLA Du, Jiangbo,et al."Enantioselective determination of ornidazole in human plasma by liquid chromatography-tandem mass spectrometry on a Chiral-AGP column".JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS 86(2013):182-188.

入库方式： OAI收割

来源：上海药物研究所

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