Metabolite profiling and identification of triptolide in rats
文献类型：期刊论文
| 作者 | Liu, Jia ; Li, Liang; Zhou, Xin; Chen, Xiaoyan; Huang, Haihua; Zhao, Shunbo; Li, Xiuli; Zhong, Dafang
|
| 刊名 | JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
 |
| 出版日期 | 2013-11-15 |
| 卷号 | 939页码:51-58 |
| 关键词 | Triptolide Metabolite profiling High-performance liquid chromatography-online radiodetector Ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry |
| ISSN号 | 1570-0232 |
| DOI | 10.1016/j.jchromb.2013.08.015 |
| 文献子类 | Article |
| 英文摘要 | The purpose of the current study was to investigate the metabolite profile of [H-3]triptolide in rats. The separation and characterisation techniques used to identify the major metabolites were high-performance liquid chromatography-online radiodetector, ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry, and nuclear magnetic resonance. In all, 33 major metabolites were detected. The major components found in the rat plasma included the parent drug and its monohydroxy- and dihydroxy-metabolites. Reference standards for the monohydroxy-metabolites were obtained either by the incubation of the parent drug with rat liver microsomes or by microbial transformation with Cunninghamella blakesleana. The metabolites' structures were identified as 17-hydroxytriptolide, 16-hydroxytriptolide, tripdiolide, and 15-hydroxytriptolide. The major metabolites found in male rat urine included the monohydroxy-, dihydroxy-, and trihydroxy-metabolites. The major metabolites in female rat urine were the monohydroxy- and dihydroxy-metabolites, as well as sulphates of the monohydroxy-metabolites. A glutathione adduct, multiple hydroxy-metabolites, and a number of unidentified metabolites were detected in the bile and faeces of male rats. Sulphates of monohydroxy-metabolites were detected in the bile and faeces of female rats. (C) 2013 Elsevier B.V. All rights reserved. |
| WOS关键词 | WILFORDII HOOK-F ; CANCER-CELLS ; IN-VITRO ; TOXICITY ; EXPRESSION |
| 资助项目 | National Natural Science Foundation of China[30901831] ; National Natural Science Foundation of China[81373479] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000326010400008 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/277373]  |
| 专题 | 上海药物代谢研究中心 |
| 通讯作者 | Zhong, Dafang |
| 作者单位 | Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Jia,Li, Liang,Zhou, Xin,et al. Metabolite profiling and identification of triptolide in rats[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2013,939:51-58. |
| APA | Liu, Jia.,Li, Liang.,Zhou, Xin.,Chen, Xiaoyan.,Huang, Haihua.,...&Zhong, Dafang.(2013).Metabolite profiling and identification of triptolide in rats.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,939,51-58. |
| MLA | Liu, Jia,et al."Metabolite profiling and identification of triptolide in rats".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 939(2013):51-58. |
入库方式： OAI收割
来源：上海药物研究所
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