Sensitive assay for measurement of volatile borneol, isoborneol, and the metabolite camphor in rat pharmacokinetic study of Borneolum (Bingpian) and Borneolum syntheticum (synthetic Bingpian)
文献类型：期刊论文
| 作者 | Cheng, Chen2; Liu, Xin-wei2; Du, Fei-fei2; Li, Mei-juan2; Xu, Fang2; Wang, Feng-qing2; Liu, Yang2; Li, Chuan1,2 ; Sun, Yan2
|
| 刊名 | ACTA PHARMACOLOGICA SINICA
 |
| 出版日期 | 2013-10 |
| 卷号 | 34期号:10页码:1337-1348 |
| 关键词 | pharmacokinetics borneol isoborneol camphor Bingpian liquid-liquid microextraction gas chromatography/mass spectrometry large-volume injection |
| ISSN号 | 1671-4083 |
| DOI | 10.1038/aps.2013.86 |
| 文献子类 | Article |
| 英文摘要 | Aim: Both Borneolum (Chinese name Bingpian; dextrorotatory borneol) and Borneolum syntheticum (synthetic Bingpian; a mixture of optically inactive borneol and isoborneol) have been used for medicinal purposes in Chinese traditional medicine. The aim of this study was to develop a sensitive assay for measuring volatile ingredients borneol, isoborneol, and their metabolite camphor in pharmacokinetic study of Bingpian. Methods: Rat plasma samples were prepared using liquid-liquid microextraction: 70 mu L of plasma sample (containing 125 nmol/L naphthalene as the internal standard) was extracted with 35 mu L of n-hexane. The resulting n-hexane extract (20 mu L) was introduced into a gas chromatography/mass spectrometry system using programmable temperature vaporizing-based large-volume injection. The assay was validated to demonstrate its reliability for the intended use. Using this assay, pharmacokinetic studies of Bingpian, synthetic Bingpian, and Fufang-Danshen tablets (containing synthetic Bingpian) were conducted in rats. Results: The extraction efficiency for the analytes and the internal standard from plasma was almost constant with decrease in n-hexane-to-plasma volume ratio, thus enabling a small volume of extracting solvent to be used for sample preparation, and enhancing the assay sensitivity. The lower quantification limit for measuring borneol, isoborneol, and camphor in plasma was 0.98 nmol/L, which was 33-330 times more sensitive than those reported earlier for Bingpian and synthetic Bingpian. The applicability of the miniaturized liquid-liquid extraction technique could be extended to measure other volatile and nonvolatile medicinal compounds in biomatrices, which can be predicted according to the analytes' octanol/water distribution coefficient (logD) and acid dissociation constant (pK(a)). Conclusion: This assay is sensitive, accurate and free of matrix effects, and can be applied to pharmacokinetic studies of Bingpian, synthetic Bingpian, and Bingpian-containing herbal products. |
| WOS关键词 | CHROMATOGRAPHY-MASS SPECTROMETRY ; LARGE-VOLUME INJECTION ; GAS-CHROMATOGRAPHY ; RESPONSE ENHANCEMENT ; HERBAL MEDICINES ; ESSENTIAL OILS ; MOUSE PLASMA ; BRAIN ; TETRAMETHYLPYRAZINE ; PILLS |
| 资助项目 | National Natural Science Fundation of China for Distinguished Young Scholars[30925044] ; National Basic Research Program of China[2012CB518403] ; National Science and Technology Major Project of China "Key New Drug Creation and Manufacturing Program"[2009ZX09304-002] |
| WOS研究方向 | Chemistry ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:000325276400010 |
| 出版者 | ACTA PHARMACOLOGICA SINICA |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/277443]  |
| 专题 | 上海药物代谢研究中心 科研与新药推进处 |
| 通讯作者 | Li, Chuan |
| 作者单位 | 1.China Acad Chinese Med Sci, Inst Chinese Mat Med, Beijing 100700, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Cheng, Chen,Liu, Xin-wei,Du, Fei-fei,et al. Sensitive assay for measurement of volatile borneol, isoborneol, and the metabolite camphor in rat pharmacokinetic study of Borneolum (Bingpian) and Borneolum syntheticum (synthetic Bingpian)[J]. ACTA PHARMACOLOGICA SINICA,2013,34(10):1337-1348. |
| APA | Cheng, Chen.,Liu, Xin-wei.,Du, Fei-fei.,Li, Mei-juan.,Xu, Fang.,...&Sun, Yan.(2013).Sensitive assay for measurement of volatile borneol, isoborneol, and the metabolite camphor in rat pharmacokinetic study of Borneolum (Bingpian) and Borneolum syntheticum (synthetic Bingpian).ACTA PHARMACOLOGICA SINICA,34(10),1337-1348. |
| MLA | Cheng, Chen,et al."Sensitive assay for measurement of volatile borneol, isoborneol, and the metabolite camphor in rat pharmacokinetic study of Borneolum (Bingpian) and Borneolum syntheticum (synthetic Bingpian)".ACTA PHARMACOLOGICA SINICA 34.10(2013):1337-1348. |
入库方式： OAI收割
来源：上海药物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。