QUANTIFICATION OF LETROZOLE IN HUMAN PLASMA USING LC-()ESI-MS/MS WITH D-4-LETROZOLE AS INTERNAL STANDARD: APPLICATION IN A PHARMACOKINETIC STUDY
文献类型：期刊论文
| 作者 | Song, Jing1,2; Zhan, Yan1 ; Chen, Xiaoyan1; Zhang, Yifan1; Zhong, Dafang1,2
|
| 刊名 | JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
 |
| 出版日期 | 2013-05-01 |
| 卷号 | 36期号:13页码:1762-1776 |
| 关键词 | human plasma LC-MS MS letrozole negative ionization mode pharmacokinetics quantification |
| ISSN号 | 1082-6076 |
| DOI | 10.1080/10826076.2012.698678 |
| 文献子类 | Article |
| 英文摘要 | To investigate the pharmacokinetics of letrozole in healthy postmenopausal Chinese women, a rapid, selective, and sensitive liquid chromatographytandem mass spectrometric method in negative ionization mode was developed and validated to determine letrozole in human plasma using d(4)-letrozole as the internal standard (IS). Following protein precipitation, effective chromatographic separation was accomplished on a Capcell PAK C-18 MG column (100mmx4.6mm, 5 mu m) with a mobile phase consisting of methanol10mM ammonium acetate (65:35, v/v) at a flow rate of 0.6mL/min. The running time was 4.0min. The detection of the analyte and IS was carried out in selective reaction monitoring mode via negative electrospray ionization interface. The method was linear over concentrations ranging from 0.40ng/mL to 50.0ng/mL. The lower limit of quantification was 0.40ng/mL, which allowed the measurement of letrozole concentration up to 240hr after oral administration. The intraday and interday precisions were less than 12.9% in terms of the relative standard deviation, and the accuracy was within +/- 7.4% in terms of the relative error. Finally, this validated method was successfully applied to the clinical pharmacokinetic study after a single oral administration of 2.5mg letrozole to 20 healthy, postmenopausal Chinese women. |
| WOS关键词 | TANDEM MASS-SPECTROMETRY ; FLUORESCENCE DETECTION ; LIQUID-CHROMATOGRAPHY ; POSTMENOPAUSAL WOMEN ; METABOLITE |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000318779800002 |
| 出版者 | TAYLOR & FRANCIS INC |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/277635]  |
| 专题 | 上海药物代谢研究中心 |
| 通讯作者 | Zhong, Dafang |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China 2.Zhejiang Univ Technol, Hangzhou, Zhejiang, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Song, Jing,Zhan, Yan,Chen, Xiaoyan,et al. QUANTIFICATION OF LETROZOLE IN HUMAN PLASMA USING LC-()ESI-MS/MS WITH D-4-LETROZOLE AS INTERNAL STANDARD: APPLICATION IN A PHARMACOKINETIC STUDY[J]. JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,2013,36(13):1762-1776. |
| APA | Song, Jing,Zhan, Yan,Chen, Xiaoyan,Zhang, Yifan,&Zhong, Dafang.(2013).QUANTIFICATION OF LETROZOLE IN HUMAN PLASMA USING LC-()ESI-MS/MS WITH D-4-LETROZOLE AS INTERNAL STANDARD: APPLICATION IN A PHARMACOKINETIC STUDY.JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,36(13),1762-1776. |
| MLA | Song, Jing,et al."QUANTIFICATION OF LETROZOLE IN HUMAN PLASMA USING LC-()ESI-MS/MS WITH D-4-LETROZOLE AS INTERNAL STANDARD: APPLICATION IN A PHARMACOKINETIC STUDY".JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES 36.13(2013):1762-1776. |
入库方式： OAI收割
来源：上海药物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。