Simultaneous quantification of 22R and 22S epimers of budesonide in human plasma by ultra-high-performance liquid chromatography-tandem mass spectrometry: Application in a stereoselective pharmacokinetic study
文献类型：期刊论文
| 作者 | Lu, Youming1,2; Sun, Zuoming1; Zhang, Yifan1 ; Chen, Xiaoyan1; Zhong, Dafang1,2
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| 刊名 | JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
 |
| 出版日期 | 2013-03-15 |
| 卷号 | 921页码:27-34 |
| 关键词 | Ultra-high-performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) Budesonide epimers Stereoselective pharmacokinetics |
| ISSN号 | 1570-0232 |
| DOI | 10.1016/j.jchromb.2013.01.015 |
| 文献子类 | Article |
| 英文摘要 | Budesonide (BUD) is used as a mixture of 22R and 22S epimers for the topical treatment of asthma, rhinitis, and inflammatory bowel disease. To study stereoselectivity in the pharmacokinetics of each epimer, we developed a stereoselective and sensitive ultra-high-performance liquid chromatography-tandem mass spectrometry method for the quantitative determination of 22R and 22S epimers of BUD in human plasma. The epimers of BUD were extracted from plasma using n-hexane/dichloromethane/isopropanol (2:1:0.1, v/v/v) under alkaline conditions. Baseline separation was obtained within 7 min on an Acquity UPLC BEH C-18 (50 mm x 2.1 mm, 1.7 pm) column using an isocratic mobile phase consisting of acetonitrile/5 mM ammonium acetate/acetic acid (29:71:0.142, v/v/v) at a flow rate of 0.7 mL/min. Mass spectrometric detection was performed in a multiple reaction monitoring mode using the m/z 489 -> 357 transition for BUD epimers and the m/z 497 -> 357 transition for the internal standard d(8)-BUD epimers. Calibration curves were linear over the concentration ranges of 5.0-500 and 5.0-3000 pg/mL for 22R-BUD and 22S-BUD, respectively. The lower limit of quantification was 5.0 pg/mL for both epimers. The method was successfully applied in a pharmacokinetic study of BUD controlled-release capsules in humans. Consistent differences in the pharmacokinetics of the 22R and 22S epimers were observed, the AUC((0-infinity)) of 22S-BUD was approximately six times higher than that of 22R-BUD, and the 22S-/22R-BUD ratio of total body clearance was 0.17. (C) 2013 Elsevier B.V. All rights reserved. |
| WOS关键词 | PHARMACOLOGICAL-PROPERTIES ; GLUCOCORTICOID BUDESONIDE ; FLUTICASONE PROPIONATE ; THERAPEUTIC-EFFICACY ; DOG PLASMA ; INHALATION ; DISEASE ; SAMPLES ; ASTHMA |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000316515900005 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/277693]  |
| 专题 | 上海药物代谢研究中心 |
| 通讯作者 | Zhong, Dafang |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China 2.Zhejiang Univ Technol, Hangzhou 310014, Zhejiang, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Lu, Youming,Sun, Zuoming,Zhang, Yifan,et al. Simultaneous quantification of 22R and 22S epimers of budesonide in human plasma by ultra-high-performance liquid chromatography-tandem mass spectrometry: Application in a stereoselective pharmacokinetic study[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2013,921:27-34. |
| APA | Lu, Youming,Sun, Zuoming,Zhang, Yifan,Chen, Xiaoyan,&Zhong, Dafang.(2013).Simultaneous quantification of 22R and 22S epimers of budesonide in human plasma by ultra-high-performance liquid chromatography-tandem mass spectrometry: Application in a stereoselective pharmacokinetic study.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,921,27-34. |
| MLA | Lu, Youming,et al."Simultaneous quantification of 22R and 22S epimers of budesonide in human plasma by ultra-high-performance liquid chromatography-tandem mass spectrometry: Application in a stereoselective pharmacokinetic study".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 921(2013):27-34. |
入库方式： OAI收割
来源：上海药物研究所
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