Identification of modification sites on human serum albumin and human hemoglobin adducts with houttuynin using liquid chromatography coupled with mass spectrometry
文献类型：期刊论文
| 作者 | Deng, Zhipeng; Zhong, Dafang ; Chen, Xiaoyan
|
| 刊名 | BIOMEDICAL CHROMATOGRAPHY
 |
| 出版日期 | 2012-11 |
| 卷号 | 26期号:11页码:1377-1385 |
| 关键词 | houttuynin human serum albumin human hemoglobin LC MSn adduct types modification sites |
| ISSN号 | 0269-3879 |
| DOI | 10.1002/bmc.2708 |
| 文献子类 | Article |
| 英文摘要 | Houttuynin, a beta-keto aldehyde compound, is the major active ingredient in herba houttuyniae injection. The injection was once used as an anti-inflammatory drug associated with occasional serious hypersensitivity reactions in the clinic, which were proposed to be related to the formation of protein adducts. N alpha-Boc-lysine, FEEM and IVTNTT were used as model amino acids or peptides containing one nucleophilic residue to investigate adduct types by liquid chromatography coupled with ion trap mass spectrometry (LC/MSn) and high-resolution quadrupole time-of-flight mass spectrometry (Q-TOF MS). These adducts were respectively characterized as Schiff bases formed by 1:1 reaction of houttuynin with lysine or N-terminal residue and pyridinium adducts by 2:1 reaction. LC/MSn analysis of trypsin digests of HSA/Hb incubations with houttuynin revealed that houttuynin-modified HSA adducts were formed mainly at N-terminal amino acid and lysine residues, specifically at Lys-212, Lys-414 and Lys-525 for Schiff base adducts, and at Lys-414 and Lys-432 for pyridinium adducts, and houttuynin adducted more readily with N-terminal valine of the alpha- and beta-chains in Hb and lysine amine (Lys-62) of the beta-chain for Schiff base adducts. The results showed the direct modification of houttuynin to HSA/Hb in vitro, which was speculated to be responsible for the adverse reactions induced by houttuyniae injection. Copyright (c) 2012 John Wiley & Sons, Ltd. |
| WOS关键词 | DRUG-PROTEIN ADDUCTS ; METABOLITES ; BINDING ; CHAIN |
| 资助项目 | National Natural Science Foundation of China[90709036] ; National Natural Science Foundation of China[81173117] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:000309412500013 |
| 出版者 | WILEY-BLACKWELL |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/277897]  |
| 专题 | 上海药物代谢研究中心 |
| 通讯作者 | Chen, Xiaoyan |
| 作者单位 | Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Deng, Zhipeng,Zhong, Dafang,Chen, Xiaoyan. Identification of modification sites on human serum albumin and human hemoglobin adducts with houttuynin using liquid chromatography coupled with mass spectrometry[J]. BIOMEDICAL CHROMATOGRAPHY,2012,26(11):1377-1385. |
| APA | Deng, Zhipeng,Zhong, Dafang,&Chen, Xiaoyan.(2012).Identification of modification sites on human serum albumin and human hemoglobin adducts with houttuynin using liquid chromatography coupled with mass spectrometry.BIOMEDICAL CHROMATOGRAPHY,26(11),1377-1385. |
| MLA | Deng, Zhipeng,et al."Identification of modification sites on human serum albumin and human hemoglobin adducts with houttuynin using liquid chromatography coupled with mass spectrometry".BIOMEDICAL CHROMATOGRAPHY 26.11(2012):1377-1385. |
入库方式： OAI收割
来源：上海药物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。