Validation of a chiral liquid chromatography-tandem mass spectrometry method for the determination of pantoprazole in dog plasma
文献类型：期刊论文
| 作者 | Chen, Meixia; Xia, Yu; Ma, Zhiyu; Li, Liang ; Zhong, Dafang; Chen, Xiaoyan
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| 刊名 | JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
 |
| 出版日期 | 2012-10-01 |
| 卷号 | 906页码:85-90 |
| 关键词 | Chiral LC-MS/MS Pantoprazole Stereoselective pharmacokinetics |
| ISSN号 | 1570-0232 |
| DOI | 10.1016/j.jchromb.2012.08.019 |
| 文献子类 | Article |
| 英文摘要 | Pantoprazole (PAN), a selective proton pump inhibitor, is used clinically as a racemic mixture for the treatment of acid-related gastrointestinal disorders. To investigate its stereoselective pharmacokinetics, a chiral liquid chromatography-tandem mass spectrometry method was developed and validated to determine the pantoprazole enantiomers in dog plasma. After liquid-liquid extraction, a baseline resolution of enantiomers was achieved on an ovomucoid column using the mobile phase of methanol:acetonitrile:10 mM ammonium formate (pH 7) (10.4:2.6:87, v/v/v) at 30 degrees C within 10 min. Stable isotopically labeled (+)-d(3)-pantoprazole and (-)-d(3)-pantoprazole were used as internal standards. Acquisition of mass spectrometric data was performed in multiple reaction monitoring mode via positive atmospheric pressure chemical ionization. The method was linear in the concentration range of 20.0-10,000 ng/mL for each enantiomer using 25 mu L of dog plasma. The lower limit of quantification (LLOQ) for each enantiomer was 20.0 ng/mL. Intra- and inter-day precision ranged from 3.2% to 10.3% for (+)-pantoprazole and 3.7-10.0% for (-)-pantoprazole. Accuracy varied from -1.4% to -0.2% for (+)-pantoprazole and -1.6% to 0.8% for (-)-pantoprazole. The validated method was applied successfully for stereoselective pharmacokinetic studies of racemic pantoprazole. (C) 2012 Elsevier B.V. All rights reserved. |
| WOS关键词 | PROTON PUMP INHIBITOR ; STEREOSELECTIVE PHARMACOKINETICS ; POOR METABOLIZERS ; STATIONARY-PHASE ; S-MEPHENYTOIN ; LC-MS/MS ; ENANTIOMERS ; RATS |
| 资助项目 | National Basic Research Program of China[2009CB930300] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000309440600012 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/277922]  |
| 专题 | 上海药物代谢研究中心 |
| 通讯作者 | Chen, Xiaoyan |
| 作者单位 | Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Meixia,Xia, Yu,Ma, Zhiyu,et al. Validation of a chiral liquid chromatography-tandem mass spectrometry method for the determination of pantoprazole in dog plasma[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2012,906:85-90. |
| APA | Chen, Meixia,Xia, Yu,Ma, Zhiyu,Li, Liang,Zhong, Dafang,&Chen, Xiaoyan.(2012).Validation of a chiral liquid chromatography-tandem mass spectrometry method for the determination of pantoprazole in dog plasma.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,906,85-90. |
| MLA | Chen, Meixia,et al."Validation of a chiral liquid chromatography-tandem mass spectrometry method for the determination of pantoprazole in dog plasma".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 906(2012):85-90. |
入库方式： OAI收割
来源：上海药物研究所
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