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Characterization of ornidazole metabolites in human bile after intraveneous doses by ultraperformance liquid chromatography/quadrupole time-of-flight mass spectrometry

文献类型：期刊论文

作者Du, Jiangbo1; Deng, Pan1; Chen, Xiaoyan1; Wang, Haidong1; You, Tiangeng2; Zhong, Dafang1
刊名ACTA PHARMACEUTICA SINICA B
出版日期2012-04
卷号2期号:2页码:159-167
关键词Ornidazole UPLC/Q-TOF MS LC-MS/MS Human bile Metabolite
ISSN号2211-3835
DOI10.1016/j.apsb.2012.01.002
文献子类Article
英文摘要Ultraperformance liquid chromatography/quadrupole time-of-flight mass spectrometry (UPLC/Q-TOF MS) was used to characterize ornidazole metabolites in human bile after intravenous doses. A liquid chromatography tandem mass spectrometry (LC MS/MS) assay was developed for the determination of the bile level of ornidazole. Bile samples, collected from four patients with T-tube drainage after biliary tract surgery, were prepared by protein precipitation with acetonitrile before analysis. A total of 12 metabolites, including 10 novel metabolites, were detected and characterized. The metabolites of ornidazole in human bile were the products of hydrochloride (HC1) elimination, oxidative dechlorination, hydroxylation, sulfation, diastereoisomeric glucuronation, and substitution of NO2 or Cl atom by cysteine or N-acetylcysteine, and oxidative dechlorination followed by further carboxylation. The bile levels of ornidazole at 12 h after multiple intravenous infusions were well above its minimal inhibitory concentration for common strains of anaerobic bacteria. (C) 2012 Institute of Materia Medica, Chinese Academy of Medical Sciences and Chinese Pharmaceutical Association. Production and hosting by Elsevier B.V. All rights reserved.
资助项目National Natural Science Foundation of China[81173117]
WOS研究方向Pharmacology & Pharmacy
语种英语
WOS记录号WOS:000413436700010
出版者INST MATERIA MEDICA, CHINESE ACAD MEDICAL SCIENCES
源URL[http://119.78.100.183/handle/2S10ELR8/278144]  
专题上海药物代谢研究中心
通讯作者You, Tiangeng
作者单位1.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China;
2.Shanghai East Hosp, Shanghai 200120, Peoples R China
推荐引用方式
GB/T 7714		 Du, Jiangbo,Deng, Pan,Chen, Xiaoyan,et al. Characterization of ornidazole metabolites in human bile after intraveneous doses by ultraperformance liquid chromatography/quadrupole time-of-flight mass spectrometry[J]. ACTA PHARMACEUTICA SINICA B,2012,2(2):159-167.
APA Du, Jiangbo,Deng, Pan,Chen, Xiaoyan,Wang, Haidong,You, Tiangeng,&Zhong, Dafang.(2012).Characterization of ornidazole metabolites in human bile after intraveneous doses by ultraperformance liquid chromatography/quadrupole time-of-flight mass spectrometry.ACTA PHARMACEUTICA SINICA B,2(2),159-167.
MLA Du, Jiangbo,et al."Characterization of ornidazole metabolites in human bile after intraveneous doses by ultraperformance liquid chromatography/quadrupole time-of-flight mass spectrometry".ACTA PHARMACEUTICA SINICA B 2.2(2012):159-167.

入库方式： OAI收割

来源：上海药物研究所

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