Development and validation of a sensitive LC-MS/MS assay for simultaneous quantitation of ranolazine and its three metabolites in human plasma
文献类型：期刊论文
| 作者 | Wang, Yuan1,3; Chen, Xiaoyan1 ; Sun, Zuoming1; Yang, Yong1; Zhang, Ying2; Liu, Wanhui3; Zhong, Dafang1
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| 刊名 | JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
 |
| 出版日期 | 2012-03-15 |
| 卷号 | 889页码:10-16 |
| 关键词 | Liquid chromatography-tandem mass spectrometry Ranolazine Metabolites Pharmacokinetics |
| ISSN号 | 1570-0232 |
| DOI | 10.1016/j.jchromb.2012.01.003 |
| 文献子类 | Article |
| 英文摘要 | A rapid, sensitive and reliable LC-MS/MS method was developed and validated for the simultaneous determination of ranolazine and its three metabolites, CVT-2514, CVT-2738, and CVT-4786, in human plasma. The plasma samples were prepared by protein precipitation. Chromatographic separation was achieved on a Gemini C-18 column (50 mm X 2.0 mm, 5 mu m) using methanol: 5 mM ammonium acetate as the mobile phase with gradient elution. Mass detection was carried out by electrospray ionization in both positive and negative ion multiple reaction monitoring (MRM) modes. The calibration curves were linear over a concentration range of 4-2000 ng/mL for ranolazine, 4-1000 ng/mL for CVT-2514 and CVT-2738 and 8-1000 ng/mL for CVT-4786. The intra-day and inter-day accuracy and precision were within the acceptable limits of +/-15% at all concentrations. The method was successfully applied for the simultaneous estimation of ranolazine and its three metabolites in human plasma from a clinical pharmacokinetics study. (C) 2012 Elsevier B.V. All rights reserved. |
| WOS关键词 | TANDEM MASS-SPECTROMETRY ; LIQUID ; IONIZATION |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000302452600003 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/278152]  |
| 专题 | 上海药物代谢研究中心 |
| 通讯作者 | Zhong, Dafang |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China; 2.Fujian Fukang Pharmaceut Co Ltd, Fuzhou 350002, Fujian, Peoples R China 3.Yantai Univ, Sch Pharm, Yantai 264005, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Wang, Yuan,Chen, Xiaoyan,Sun, Zuoming,et al. Development and validation of a sensitive LC-MS/MS assay for simultaneous quantitation of ranolazine and its three metabolites in human plasma[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2012,889:10-16. |
| APA | Wang, Yuan.,Chen, Xiaoyan.,Sun, Zuoming.,Yang, Yong.,Zhang, Ying.,...&Zhong, Dafang.(2012).Development and validation of a sensitive LC-MS/MS assay for simultaneous quantitation of ranolazine and its three metabolites in human plasma.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,889,10-16. |
| MLA | Wang, Yuan,et al."Development and validation of a sensitive LC-MS/MS assay for simultaneous quantitation of ranolazine and its three metabolites in human plasma".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 889(2012):10-16. |
入库方式： OAI收割
来源：上海药物研究所
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