Nebulizing conditions of pneumatic electrospray ionization significantly influence electrolyte effects on compound measurement
文献类型：期刊论文
| 作者 | Niu, Wei1; Zhu, Xiaohong1,2; Yu, Ke1; Li, Li1; Sun, Yan1; Li, Chuan1,2
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| 刊名 | JOURNAL OF MASS SPECTROMETRY
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| 出版日期 | 2012-03 |
| 卷号 | 47期号:3页码:370-380 |
| 关键词 | electrospray ionization electrolyte effects signal enhancement matrix effects upper limit of quantification ginkgo terpene lactone ginkgo flavonol |
| ISSN号 | 1076-5174 |
| DOI | 10.1002/jms.2050 |
| 文献子类 | Article |
| 英文摘要 | Composition of mobile phase can greatly influence the success of electrospray ionization (ESI)-interfaced liquid chromatographymass spectrometry analysis. To investigate the relationship between formic-acid-based modification of mobile phase and ESI nebulizing conditions, an API 4000 ESI source and a TSQ Quantum one were compared under the same chromatographic conditions. Ginkgo terpene lactones and flavonols were measured in plasma, which involved using ascorbic acid to circumvent cross-interference between the analytes. ESI responses to using formic acid included changes in signal intensity, matrix effect, and upper limit of quantification. Significant disparities in the responses were observed between the two ESI sources, suggesting that the use of electrolyte modifier in liquid chromatography mobile phase and the pneumatic nebulization for ESI should be properly balanced to accomplish optimal ESI-based analysis. The distribution of unpaired ions toward the surface of the initial droplet was assumed to be an important step in the pneumatic ESI process. When using the electrolyte in mobile phase, a too fast droplet reduction by rapid-heating-assisted pneumatic nebulization could negatively decrease the time available for the unpaired ions to migrate from droplet interior to its surface. Ascorbic acid was identified as a major interfering substance for the bioanalytical assay; the interference mechanism might be associated with hindering the unpaired analyte ions from distributing toward the droplet surface rather than outcompeting the analyte ions for the limited excess charge on droplets surface. The current work extends the knowledge base of pneumatic ESI, which has implication for optimal use of the ESI-interfaced liquid chromatographymass spectrometry technique. Copyright (C) 2012 John Wiley & Sons, Ltd. |
| WOS关键词 | CHROMATOGRAPHY-MASS SPECTROMETRY ; LASER-INDUCED FLUORESCENCE ; ELUENT COMPOSITION ; DRUG DISCOVERY ; PERFORMANCE ; PLASMA ; METABOLITES ; EFFICIENCY ; RETENTION ; DROPLETS |
| 资助项目 | National Science Fund of China for Distinguished Young Scholars[30925044] ; National Science and Technology Major Project of China "Key New Drug Creation and Manufacturing Program"[2009ZX09304-002] ; National Natural Science Foundation of China[3077277] ; National Basic Research Program of China[2012CB518403] ; Shanghai Science and Technology Major Projects[07DZ19703] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry ; Spectroscopy |
| 语种 | 英语 |
| WOS记录号 | WOS:000301575200014 |
| 出版者 | WILEY-BLACKWELL |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/278163]  |
| 专题 | 上海药物代谢研究中心 科研与新药推进处 |
| 通讯作者 | Li, Chuan |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, Lab DMPK Res Herbal Med, Shanghai 201203, Peoples R China; 2.Chinese Acad Sci, Grad Sch, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Niu, Wei,Zhu, Xiaohong,Yu, Ke,et al. Nebulizing conditions of pneumatic electrospray ionization significantly influence electrolyte effects on compound measurement[J]. JOURNAL OF MASS SPECTROMETRY,2012,47(3):370-380. |
| APA | Niu, Wei,Zhu, Xiaohong,Yu, Ke,Li, Li,Sun, Yan,&Li, Chuan.(2012).Nebulizing conditions of pneumatic electrospray ionization significantly influence electrolyte effects on compound measurement.JOURNAL OF MASS SPECTROMETRY,47(3),370-380. |
| MLA | Niu, Wei,et al."Nebulizing conditions of pneumatic electrospray ionization significantly influence electrolyte effects on compound measurement".JOURNAL OF MASS SPECTROMETRY 47.3(2012):370-380. |
入库方式： OAI收割
来源：上海药物研究所
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