Trimethylsilyldiazomethane derivatization coupled with solid-phase extraction for the determination of alendronate in human plasma by LC-MS/MS
文献类型：期刊论文
| 作者 | Chen, Meixia; Liu, Ke; Zhong, Dafang ; Chen, Xiaoyan
|
| 刊名 | ANALYTICAL AND BIOANALYTICAL CHEMISTRY
 |
| 出版日期 | 2012-01 |
| 卷号 | 402期号:2页码:791-798 |
| 关键词 | Alendronate Derivatization LC-MS/MS Trimethylsilyldiazomethane Pharmacokinetics |
| ISSN号 | 1618-2642 |
| DOI | 10.1007/s00216-011-5467-4 |
| 文献子类 | Article |
| 英文摘要 | Alendronate is an important representative of bisphosphonates, strongly polar compounds that lack chromophores. With rare exceptions, derivatization of the analytes is necessary for bioanalysis. In this study, a rapid liquid chromatography-tandem mass spectrometry method employing pre-column derivatization was developed and validated for the determination of alendronate concentrations in human plasma. The procedure was based on derivatization with trimethylsilyldiazomethane during solid-phase extraction on a weak anion-exchange solid-phase cartridge, which integrated sample purification and derivatization into one step. The alendronate derivative was eluted with methanol. Chromatographic separation was performed on a Capcell PAK-C18 column. The total run time was 6.5 min. The calibration curve was linear in the range 1.00-1,000 ng/mL using d6-alendronate as the internal standard. The lower limit of quantification was 1.00 ng/mL. The intra- and inter-assay precision (in RSD) calculated from quality control samples was less than 15%, and the accuracy was between 98.1% and 100.2%. The validated method was successfully applied to characterize the pharmacokinetic profiles of alendronate following the intravenous infusion of 5 or 10 mg alendronate sodium to healthy volunteers. |
| WOS关键词 | PERFORMANCE LIQUID-CHROMATOGRAPHY ; HUMAN URINE ; ION CHROMATOGRAPHY ; MASS-SPECTROMETRY ; FLUORESCENCE DETECTION ; BISPHOSPHONATES ; SODIUM ; OSTEOPOROSIS ; WOMEN |
| 资助项目 | National Basic Research Program of China[2009CB930300] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000298645300021 |
| 出版者 | SPRINGER HEIDELBERG |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/278284]  |
| 专题 | 上海药物代谢研究中心 |
| 通讯作者 | Chen, Xiaoyan |
| 作者单位 | Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Meixia,Liu, Ke,Zhong, Dafang,et al. Trimethylsilyldiazomethane derivatization coupled with solid-phase extraction for the determination of alendronate in human plasma by LC-MS/MS[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2012,402(2):791-798. |
| APA | Chen, Meixia,Liu, Ke,Zhong, Dafang,&Chen, Xiaoyan.(2012).Trimethylsilyldiazomethane derivatization coupled with solid-phase extraction for the determination of alendronate in human plasma by LC-MS/MS.ANALYTICAL AND BIOANALYTICAL CHEMISTRY,402(2),791-798. |
| MLA | Chen, Meixia,et al."Trimethylsilyldiazomethane derivatization coupled with solid-phase extraction for the determination of alendronate in human plasma by LC-MS/MS".ANALYTICAL AND BIOANALYTICAL CHEMISTRY 402.2(2012):791-798. |
入库方式： OAI收割
来源：上海药物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。