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Quantifying tetrapeptide SS-20 in rat plasma using hydrophilic interaction liquid chromatography coupled with electrospray ionization tandem mass spectrometry

文献类型：期刊论文

作者Zhan, Yan; Chen, Xiaoyan; Zhong, Dafang
刊名JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
出版日期2011-11-15
卷号879期号:30页码:3353-3359
关键词Hydrophilic interaction liquid chromatography Liquid chromatography tandem mass spectrometry Peptide Pharmacokinetics
ISSN号1570-0232
DOI10.1016/j.jchromb.2011.06.033
文献子类Article
英文摘要Quantitative analysis of peptides in biological matrices remains a challenging task. This is due to the low dosage and the complexity of both the matrix and the analytical characteristics of peptides. SS-20 is a tetrapeptide compound developed for the treatment of Parkinson's disease. To investigate the pharmacokinetics of SS-20, a sensitive and rapid liquid chromatography coupled with mass spectrometry method was developed and validated. An aliquot of 50 mu L plasma sample was extracted via solid phase extraction. The extracts were separated using a hydrophilic interaction liquid chromatography column, and were then detected with a triple quadrupole mass spectrometer using electrospray ionization in positive-ion mode and selected reaction monitoring. The use of a deuterium-labeled internal standard provided acceptable accuracy, precision, and matrix effect. The lower limit of quantification was 0.30 ng/mL. The linear range of the method was from 0.30 to 1000 ng/mL. The intraday and interday precisions were lower than 10.2% in terms of relative standard deviation, and the accuracy was within +/- 2.1% in terms of relative error. The validated LC-MS/MS method was successfully applied to a pharmacokinetic study of SS-20 following an intravenous or subcutaneous injection administration of 1.0 mg/kg to Sprague-Dawley rats. (C) 2011 Elsevier B.V. All rights reserved.
WOS关键词IN-OVINE PLASMA ; PEPTIDES ; QUANTIFICATION ; SEPARATION ; ADSORPTION
资助项目National Natural Sciences Foundation of China[30472053]
WOS研究方向Biochemistry & Molecular Biology ; Chemistry
语种英语
WOS记录号WOS:000297396200001
出版者ELSEVIER SCIENCE BV
源URL[http://119.78.100.183/handle/2S10ELR8/278334]  
专题上海药物代谢研究中心
通讯作者Zhong, Dafang
作者单位Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China
推荐引用方式
GB/T 7714		 Zhan, Yan,Chen, Xiaoyan,Zhong, Dafang. Quantifying tetrapeptide SS-20 in rat plasma using hydrophilic interaction liquid chromatography coupled with electrospray ionization tandem mass spectrometry[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2011,879(30):3353-3359.
APA Zhan, Yan,Chen, Xiaoyan,&Zhong, Dafang.(2011).Quantifying tetrapeptide SS-20 in rat plasma using hydrophilic interaction liquid chromatography coupled with electrospray ionization tandem mass spectrometry.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,879(30),3353-3359.
MLA Zhan, Yan,et al."Quantifying tetrapeptide SS-20 in rat plasma using hydrophilic interaction liquid chromatography coupled with electrospray ionization tandem mass spectrometry".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 879.30(2011):3353-3359.

入库方式： OAI收割

来源：上海药物研究所

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