Quantifying tetrapeptide SS-20 in rat plasma using hydrophilic interaction liquid chromatography coupled with electrospray ionization tandem mass spectrometry
文献类型:期刊论文
作者 | Zhan, Yan![]() ![]() ![]() |
刊名 | JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
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出版日期 | 2011-11-15 |
卷号 | 879期号:30页码:3353-3359 |
关键词 | Hydrophilic interaction liquid chromatography Liquid chromatography tandem mass spectrometry Peptide Pharmacokinetics |
ISSN号 | 1570-0232 |
DOI | 10.1016/j.jchromb.2011.06.033 |
文献子类 | Article |
英文摘要 | Quantitative analysis of peptides in biological matrices remains a challenging task. This is due to the low dosage and the complexity of both the matrix and the analytical characteristics of peptides. SS-20 is a tetrapeptide compound developed for the treatment of Parkinson's disease. To investigate the pharmacokinetics of SS-20, a sensitive and rapid liquid chromatography coupled with mass spectrometry method was developed and validated. An aliquot of 50 mu L plasma sample was extracted via solid phase extraction. The extracts were separated using a hydrophilic interaction liquid chromatography column, and were then detected with a triple quadrupole mass spectrometer using electrospray ionization in positive-ion mode and selected reaction monitoring. The use of a deuterium-labeled internal standard provided acceptable accuracy, precision, and matrix effect. The lower limit of quantification was 0.30 ng/mL. The linear range of the method was from 0.30 to 1000 ng/mL. The intraday and interday precisions were lower than 10.2% in terms of relative standard deviation, and the accuracy was within +/- 2.1% in terms of relative error. The validated LC-MS/MS method was successfully applied to a pharmacokinetic study of SS-20 following an intravenous or subcutaneous injection administration of 1.0 mg/kg to Sprague-Dawley rats. (C) 2011 Elsevier B.V. All rights reserved. |
WOS关键词 | IN-OVINE PLASMA ; PEPTIDES ; QUANTIFICATION ; SEPARATION ; ADSORPTION |
资助项目 | National Natural Sciences Foundation of China[30472053] |
WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
语种 | 英语 |
WOS记录号 | WOS:000297396200001 |
出版者 | ELSEVIER SCIENCE BV |
源URL | [http://119.78.100.183/handle/2S10ELR8/278334] ![]() |
专题 | 上海药物代谢研究中心 |
通讯作者 | Zhong, Dafang |
作者单位 | Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China |
推荐引用方式 GB/T 7714 | Zhan, Yan,Chen, Xiaoyan,Zhong, Dafang. Quantifying tetrapeptide SS-20 in rat plasma using hydrophilic interaction liquid chromatography coupled with electrospray ionization tandem mass spectrometry[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2011,879(30):3353-3359. |
APA | Zhan, Yan,Chen, Xiaoyan,&Zhong, Dafang.(2011).Quantifying tetrapeptide SS-20 in rat plasma using hydrophilic interaction liquid chromatography coupled with electrospray ionization tandem mass spectrometry.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,879(30),3353-3359. |
MLA | Zhan, Yan,et al."Quantifying tetrapeptide SS-20 in rat plasma using hydrophilic interaction liquid chromatography coupled with electrospray ionization tandem mass spectrometry".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 879.30(2011):3353-3359. |
入库方式: OAI收割
来源:上海药物研究所
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