Simultaneous determination of dronedarone and its active metabolite debutyldronedarone in human plasma by liquid chromatography-tandem mass spectrometry: Application to a pharmacokinetic study
文献类型：期刊论文
| 作者 | Xie, Cen ; Yang, Shilei; Zhong, Dafang; Dai, Xiaojian; Chen, Xiaoyan
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| 刊名 | JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
 |
| 出版日期 | 2011-10-15 |
| 卷号 | 879期号:28页码:3071-3075 |
| 关键词 | Liquid chromatography tandem mass spectrometry Dronedarone Debutyldronedarone Pharmacokinetics |
| ISSN号 | 1570-0232 |
| DOI | 10.1016/j.jchromb.2011.09.004 |
| 文献子类 | Article |
| 英文摘要 | Dronedarone is a derivative of amiodarone - a popular antiarrhythmic drug. It was developed to overcome the limiting iodine-associated toxicities of amiodarone. Debutyldronedarone is a major circulating active metabolite of dronedarone in humans. To investigate the pharmacokinetics of dronedarone. a rapid, simple, and sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and validated to simultaneously determine dronedarone and debutyldronedarone in human plasma using arniodarone as internal standard (IS). Acetonitrile with IS was used to precipitate proteins from a 50-mu L aliquot of plasma. Effective chromatographic separation was performed on a CAPCELL PAK C(18) MG (100 mm x 4.6 mm, 5 mu m) column with gradient elution (5 mmol/L ammonium acetate-acetonitrile, with each phase containing 0.2% acetic acid) at a flow rate of 0.7 mL/min. Complete separation was achieved within 5.5 min. Detection was carried out on an tandem mass spectrometer in multiple reaction monitoring mode using a positive atmospheric pressure chemical ionization interface. A lower limit of quantification of 0.200 ng/mL was achieved for both dronedarone and debutyldronedarone, with acceptable precision and accuracy. The linear range of the method was from 0.200 to 200 ng/mL for each analyte. Intra- and inter-day precisions were lower than 7.2% in relation to relative standard deviation, while accuracy was within +/- 5.1% in terms of relative error for analytes. Our findings demonstrate the successful application of the validated LC-MS/MS method to a pharmacokinetic study after a single oral administration of 400 mg dronedarone to six healthy volunteers. (C) 2011 Elsevier B.V. All rights reserved. |
| WOS关键词 | ATRIAL-FIBRILLATION |
| 资助项目 | National Basic Research Program of China[2009CB930300] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000296073800021 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/278371]  |
| 专题 | 上海药物代谢研究中心 |
| 通讯作者 | Chen, Xiaoyan |
| 作者单位 | Chinese Acad Sci, Ctr Drug Metab & Pharmacokinet, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xie, Cen,Yang, Shilei,Zhong, Dafang,et al. Simultaneous determination of dronedarone and its active metabolite debutyldronedarone in human plasma by liquid chromatography-tandem mass spectrometry: Application to a pharmacokinetic study[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2011,879(28):3071-3075. |
| APA | Xie, Cen,Yang, Shilei,Zhong, Dafang,Dai, Xiaojian,&Chen, Xiaoyan.(2011).Simultaneous determination of dronedarone and its active metabolite debutyldronedarone in human plasma by liquid chromatography-tandem mass spectrometry: Application to a pharmacokinetic study.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,879(28),3071-3075. |
| MLA | Xie, Cen,et al."Simultaneous determination of dronedarone and its active metabolite debutyldronedarone in human plasma by liquid chromatography-tandem mass spectrometry: Application to a pharmacokinetic study".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 879.28(2011):3071-3075. |
入库方式： OAI收割
来源：上海药物研究所
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