HPLC-MS-MS for the Simultaneous Determination of Atorvastatin and Amlodipine in Plasma of Hypertensive Patients
文献类型：期刊论文
| 作者 | Yu, Qi3; Hu, Zhe-Yi2; Zhu, Fan-Yuan1; Zhu, Jin-Hui3; Wan, Li-Li3; Li, Yan3; Guo, Cheng3 |
| 刊名 | CHROMATOGRAPHIA
 |
| 出版日期 | 2011-02 |
| 卷号 | 73期号:3-4页码:257-262 |
| 关键词 | High performance liquid chromatography Tandem mass spectrometry Atorvastatin Amlodipine Plasma samples |
| ISSN号 | 0009-5893 |
| DOI | 10.1007/s10337-010-1883-4 |
| 文献子类 | Article |
| 英文摘要 | Combination drug products containing amlodipine and atorvastatin are widely marketed and used in the treatment of concomitant hypertension and dyslipidemia. A rapid, simple and sensitive high performance liquid chromatography-tandem mass spectrometry (HPLC-MS-MS) method for determination of atorvastatin and amlodipine in plasma of hypertensive patients has been developed and validated to be used for therapeutic drug monitoring. The plasma samples were subjected to methanol protein precipitation. Chromatographic separation was performed on a C18 column using a gradient elution. The mobile phase consisted of 0.1% of formic acid in water and 0.1% of formic acid in acetonitrile and was pumped at a flow rate of 0.4 mL min(-1). Detection of analytes was achieved by tandem mass spectrometry with electrospray ionization (ESI) interface in positive ion mode. The calibration curves were linear over the range of 0.46-1,000 ng mL(-1). The intra- and inter-day precisions were within 12.2%, while the accuracy ranged from 92.7 to 108.1%. The validated LC-MS-MS method was successfully applied for the determination of atorvastatin and amlodipine in plasma of hypertensive patients. |
| WOS关键词 | BIOEQUIVALENCE EVALUATION ; MS/MS METHOD ; OPEN-LABEL ; SIMVASTATIN ; COMBINATION ; PHARMACOKINETICS ; QUANTITATION ; FORMULATIONS ; CROSSOVER ; DISEASE |
| 资助项目 | School of Medicine of Shanghai Jiao Tong University[JYY0909] ; Shanghai Sixth People's Hospital (Shanghai, China)[00000000] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000287204000007 |
| 出版者 | SPRINGER HEIDELBERG |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/278610]  |
| 专题 | 上海药物代谢研究中心 |
| 通讯作者 | Guo, Cheng |
| 作者单位 | 1.Second Mil Med Univ, Changzheng Hosp, Ctr Renal Transplantat, Shanghai, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai Ctr DMPK Res, Shanghai 200031, Peoples R China; 3.Shanghai Jiao Tong Univ, Shanghai Peoples Hosp 6, Dept Pharm, Shanghai 200030, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Yu, Qi,Hu, Zhe-Yi,Zhu, Fan-Yuan,et al. HPLC-MS-MS for the Simultaneous Determination of Atorvastatin and Amlodipine in Plasma of Hypertensive Patients[J]. CHROMATOGRAPHIA,2011,73(3-4):257-262. |
| APA | Yu, Qi.,Hu, Zhe-Yi.,Zhu, Fan-Yuan.,Zhu, Jin-Hui.,Wan, Li-Li.,...&Guo, Cheng.(2011).HPLC-MS-MS for the Simultaneous Determination of Atorvastatin and Amlodipine in Plasma of Hypertensive Patients.CHROMATOGRAPHIA,73(3-4),257-262. |
| MLA | Yu, Qi,et al."HPLC-MS-MS for the Simultaneous Determination of Atorvastatin and Amlodipine in Plasma of Hypertensive Patients".CHROMATOGRAPHIA 73.3-4(2011):257-262. |
入库方式： OAI收割
来源：上海药物研究所
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