Enantioselective determination of doxazosin in human plasma by liquid chromatography-tandem mass spectrometry using ovomucoid chiral stationary phase
文献类型：期刊论文
| 作者 | Liu, Ke; Zhong, Dafang ; Chen, Xiaoyan
|
| 刊名 | JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
 |
| 出版日期 | 2010-09-15 |
| 卷号 | 878期号:26页码:2415-2420 |
| 关键词 | Doxazosin Enantiomers Enantioselective LC-MS/MS Stereoselective pharmacokinetics |
| ISSN号 | 1570-0232 |
| DOI | 10.1016/j.jchromb.2010.07.027 |
| 文献子类 | Article |
| 英文摘要 | An enantioselective and sensitive method was developed and validated for determination of cloxazosin enantiomers in human plasma by liquid chromatography-tandem mass spectrometry. The enantiomers of doxazosin were extracted from plasma using ethyl ether/dichloromethane (3/2, v/v) under alkaline conditions. Baseline chiral separation was obtained within 9 min on an ovomucoid column using an isocratic mobile phase of methanol/5 mM ammonium acetate/formic acid (20/80/0.016, v/v/v) at a flow rate of 0.60 mL/min. Acquisition of mass spectrometric data was performed in multiple reaction monitoring mode, using the transitions of m/z 452 -> 0344 for doxazosin enantiomers, and m/z 384 -> 247 for prazosin (internal standard). The method was linear in the concentration range of 0.100-50.0 ng/mL for each enantiomer using 200 mu L of plasma. The lower limit of quantification (LLOQ) for each enantiomer was 0.100 ng/mL. The intra- and inter-assay precision was 5.0-11.1% and 5.7-7.6% for R-(-)-doxazosin and S-(+)-doxazosin, respectively. The accuracy was 97.4-99.5% for R-(-)-doxazosin and 96.8-102.8% for S-(+)-doxazosin. No chiral inversion was observed during the plasma storage, preparation and analysis. The method proved adequate for enantioselective pharmacokinetic studies of doxazosin after oral administration of therapeutic doses of racemic doxazosin. (C) 2010 Elsevier B.V. All rights reserved. |
| WOS关键词 | BENIGN PROSTATIC HYPERPLASIA ; QUANTIFICATION ; COMBINATION ; VALIDATION ; SWITCHES ; TABLETS ; MS/MS |
| 资助项目 | National Basic Research Program of China[2009CB930300] ; Science and Technology Commission of Shanghai Municipality[08DZ1980200] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000282079100008 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/278775]  |
| 专题 | 上海药物代谢研究中心 |
| 通讯作者 | Chen, Xiaoyan |
| 作者单位 | Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Ke,Zhong, Dafang,Chen, Xiaoyan. Enantioselective determination of doxazosin in human plasma by liquid chromatography-tandem mass spectrometry using ovomucoid chiral stationary phase[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2010,878(26):2415-2420. |
| APA | Liu, Ke,Zhong, Dafang,&Chen, Xiaoyan.(2010).Enantioselective determination of doxazosin in human plasma by liquid chromatography-tandem mass spectrometry using ovomucoid chiral stationary phase.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,878(26),2415-2420. |
| MLA | Liu, Ke,et al."Enantioselective determination of doxazosin in human plasma by liquid chromatography-tandem mass spectrometry using ovomucoid chiral stationary phase".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 878.26(2010):2415-2420. |
入库方式： OAI收割
来源：上海药物研究所
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