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Determination of picamilon concentration in human plasma by liquid chromatography-tandem mass spectrometry

文献类型：期刊论文

作者Cui, Wenqi1,2; Chen, Xiaoyan2; Zhan, Yan2; Zhang, Zhenzhong1; Zhang, Yifan2; Zhong, Dafang2
刊名JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
出版日期2010-05-01
卷号878期号:15-16页码:1181-1184
关键词Picamilon LC-MS/MS Pharmacokinetics Human plasma
ISSN号1570-0232
DOI10.1016/j.jchromb.2010.03.013
文献子类Article
英文摘要A rapid liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and validated for the determination of picamilon concentration in human plasma. Picamilon was extracted from human plasma by protein precipitation. High performance liquid chromatography separation was performed on a Venusil ASB C-18 column with a mobile phase consisting of methanol -10 mM ammonium acetate-formic acid (55:45:01, v/v/v) at a flow rate of 0.65 ml/min. Acquisition of mass spectrometric data was performed in selected reaction monitoring mode, using the transitions of m/z 209.0 -> m/z (78.0 + 106.0) for picamilon and m/z 152.0 -> m/z (93.0 + 110.0) for paracetamol (internal standard). The method was linear in the concentration range of 1.00-5000 ng/ml for the analyte. The lower limit of quantification was 1.00 ng/ml. The intra- and inter-assay precision were below 13.5%, and the accuracy was between 99.6% and 101.6%. The method was successfully applied to characterize the pharmacokinetic profiles of picamilon in healthy volunteers. This validated LC-MS/MS method was selective and rapid, and is suitable for the pharmacokinetic study of picamilon in humans. (C) 2010 Elsevier B.V. All rights reserved.
资助项目National Basic Research Program of China[2009CB930300]
WOS研究方向Biochemistry & Molecular Biology ; Chemistry
语种英语
WOS记录号WOS:000277672000021
出版者ELSEVIER SCIENCE BV
源URL[http://119.78.100.183/handle/2S10ELR8/278911]  
专题上海药物代谢研究中心
通讯作者Zhong, Dafang
作者单位1.Zhengzhou Univ, Zhengzhou 450001, Peoples R China
2.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China;
推荐引用方式
GB/T 7714		 Cui, Wenqi,Chen, Xiaoyan,Zhan, Yan,et al. Determination of picamilon concentration in human plasma by liquid chromatography-tandem mass spectrometry[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2010,878(15-16):1181-1184.
APA Cui, Wenqi,Chen, Xiaoyan,Zhan, Yan,Zhang, Zhenzhong,Zhang, Yifan,&Zhong, Dafang.(2010).Determination of picamilon concentration in human plasma by liquid chromatography-tandem mass spectrometry.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,878(15-16),1181-1184.
MLA Cui, Wenqi,et al."Determination of picamilon concentration in human plasma by liquid chromatography-tandem mass spectrometry".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 878.15-16(2010):1181-1184.

入库方式： OAI收割

来源：上海药物研究所

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