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A simple and rapid high-performance liquid chromatography method for determination of alendronate sodium in beagle dog plasma with application to preclinical pharmacokinetic study

文献类型：期刊论文

作者Meng, Jian2; Meng, Qiang1; Zheng, Liangyuan3
刊名BIOMEDICAL CHROMATOGRAPHY
出版日期2010-02
卷号24期号:2页码:169-173
关键词alendronate sodium o-phthalaldehyde derivatization plasma pharmacokinetics
ISSN号0269-3879
DOI10.1002/bmc.1266
文献子类Article
英文摘要A simple and rapid high performance liquid chromatographic (HPLC) method for quantifying alendronate in beagle dog plasma was developed, validated and applied to a pharmacokinetic study. The sample preparation involved coprecipitation with CaCl(2) and derivatization with o-phthalaldehyde. Chromatographic separation was achieved on a Diamonsil(TM) C(18) (250 x 4.6 mm, 5 mu m) using acetonitrile-0.4% EDTA-Na(2) (16:84, v/v) containing 0.034% of NaOH as mobile phase. The fluorimetric detector was operated at 339 nm (excitation) and 447 nm (emission). The linearity over the concentration range of 5.00-600 ng/mL for alendronate was obtained and the lower limit of quantification was 5.00 ng/mL. For each level of quality control samples, inter-day and intra-day precisions were less than 8.52 and 7.42% and accuracies were less than 9.07%. The assay was applied to the analysis of samples from a pharmacokinetic study. Following the oral administration of 70 mg alendronate sodium to beagle dogs, the maximum plasma concentration (C(max)) and elimination half-life were 152 +/- 27.3 and 1.75 +/- 0.267 h, respectively. The method was demonstrated to be highly feasible and reproducible for pharmacokinetic studies. Copyright (C) 2009 John Wiley & Sons, Ltd.
WOS关键词BISPHOSPHONATES ; FLUORESCENCE
WOS研究方向Biochemistry & Molecular Biology ; Chemistry ; Pharmacology & Pharmacy
语种英语
WOS记录号WOS:000273972600007
出版者JOHN WILEY & SONS LTD
源URL[http://119.78.100.183/handle/2S10ELR8/278978]  
专题上海药物代谢研究中心
通讯作者Meng, Jian
作者单位1.China Med Univ, Hosp 1, Shenyang 110001, Peoples R China;
2.Chinese Acad Sci, Shanghai Inst Mat Media, Shanghai 201203, Peoples R China;
3.Shenyang Pharmaceut Univ, Shenyang 110015, Peoples R China
推荐引用方式
GB/T 7714		 Meng, Jian,Meng, Qiang,Zheng, Liangyuan. A simple and rapid high-performance liquid chromatography method for determination of alendronate sodium in beagle dog plasma with application to preclinical pharmacokinetic study[J]. BIOMEDICAL CHROMATOGRAPHY,2010,24(2):169-173.
APA Meng, Jian,Meng, Qiang,&Zheng, Liangyuan.(2010).A simple and rapid high-performance liquid chromatography method for determination of alendronate sodium in beagle dog plasma with application to preclinical pharmacokinetic study.BIOMEDICAL CHROMATOGRAPHY,24(2),169-173.
MLA Meng, Jian,et al."A simple and rapid high-performance liquid chromatography method for determination of alendronate sodium in beagle dog plasma with application to preclinical pharmacokinetic study".BIOMEDICAL CHROMATOGRAPHY 24.2(2010):169-173.

入库方式： OAI收割

来源：上海药物研究所

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