Rapid and sensitive liquid chromatography-tandem mass spectrometry method for the determination of leuprolide in human serum
文献类型：期刊论文
| 作者 | Zhan, Yan ; Chen, Xiaoyan; Zhao, Xiaohua; Zhong, Dafang
|
| 刊名 | JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
 |
| 出版日期 | 2009-10-01 |
| 卷号 | 877期号:27页码:3194-3200 |
| ISSN号 | 1570-0232 |
| 关键词 | Liquid chromatography-tandem mass spectrometry Leuprolide Pharmacokinetics |
| DOI | 10.1016/j.jchromb.2009.08.023 |
| 文献子类 | Article |
| 英文摘要 | Leuprolide is a synthetic nonapeptide that has two basic amino acids, arginine and histidine, in its structure. By selection of an appropriate analytical column and optimizing the mobile phase composition, an improved analytical method has been developed and validated to determine leuprolide concentrations in human serum. After methanol-induced protein precipitation of serum samples and Oasis (R) HLB cartridge solid-phase extraction, leuprolide and an internal standard (alarelin) were analyzed on a C-18 column interfaced with a triple quadrupole tandem mass spectrometer with positive electrospray ionization. The mobile phase consisted of acetonitrile-water-propionic acid (20:80:0.05). The analyte and internal standard were both detected in the selective reaction monitoring mode. The method exhibited a linear range of 0.018-45.2 ng/mL for leuprolide. The intra- and inter-assay precisions were 11.5% or less relative standard deviation (R.S.D.), and accuracy was within +/- 2.8% relative error (RE). The lower limit of quantification (LLOQ) was identifiable and reproducible at 0.018 ng/mL, with acceptable precision and accuracy. The validated LC-MS/MS method was tested to a clinical pharmacokinetic study of leuprolide after a single subcutaneous injection of 1 mg leuprolide acetate in healthy male Chinese volunteers. (C) 2009 Elsevier B.V. All rights reserved. |
| WOS关键词 | PHARMACOKINETICS ; ACETATE ; LEUPRORELIN ; PLASMA ; PHARMACODYNAMICS ; VALIDATION ; TOXICITY |
| 资助项目 | National Natural Sciences Foundation of China[30472053] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| 出版者 | ELSEVIER SCIENCE BV |
| WOS记录号 | WOS:000270246400036 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/279117]  |
| 专题 | 上海药物代谢研究中心 |
| 通讯作者 | Zhong, Dafang |
| 作者单位 | Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhan, Yan,Chen, Xiaoyan,Zhao, Xiaohua,et al. Rapid and sensitive liquid chromatography-tandem mass spectrometry method for the determination of leuprolide in human serum[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2009,877(27):3194-3200. |
| APA | Zhan, Yan,Chen, Xiaoyan,Zhao, Xiaohua,&Zhong, Dafang.(2009).Rapid and sensitive liquid chromatography-tandem mass spectrometry method for the determination of leuprolide in human serum.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,877(27),3194-3200. |
| MLA | Zhan, Yan,et al."Rapid and sensitive liquid chromatography-tandem mass spectrometry method for the determination of leuprolide in human serum".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 877.27(2009):3194-3200. |
入库方式： OAI收割
来源：上海药物研究所
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