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Liquid chromatography/tandem mass spectrometry method for the quantification of deserpidine in human plasma: Application to a pharmacokinetic study

文献类型：期刊论文

作者Zhang, Huaicheng1,2; Zhong, Dafang2; Zhang, Zhenzhong1; Dai, Xiaojian2; Chen, Xiaoyan2
刊名JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
出版日期2009-10-01
卷号877期号:27页码:3221-3225
关键词Deserpidine Reserpine LC/MS/MS Pharmacokinetics
ISSN号1570-0232
DOI10.1016/j.jchromb.2009.06.005
文献子类Article
英文摘要A sensitive and rapid liquid chromatography/tandem mass spectrometric (LC/MS/MS) method was developed and validated for the determination of deserpidine in human plasma. The plasma samples were prepared using liquid-liquid extraction (LLE) with ethyl ether-dichloromethane (3:2, v/v). Chromatographic separation was accomplished on an Ultimate XB-C18 column. The mobile phase consisted of methanol-5 mM ammonium acetate-formic acid (72:28:0.036, v/v/v). Detection of deserpidine and the internal standard tropisetron was achieved by tandem mass spectrometry with an electrospray ionization interface in positive ion mode. The lower limit of quantification was 4.0 pg/ml. The linear range of the method was from 4.0 to 2000 pg/ml. The intra- and inter-day precisions were lower than 14.7% in terms of relative standard deviation (RSD), and the accuracy was within +/- 8.7% in terms of relative error (RE). This validated method was successfully applied for the evaluation of pharmacokinetics of deserpidine after a single oral administration dose of 0.25 mg deserpidine to 22 healthy volunteers. (C) 2009 Elsevier B.V. All rights reserved.
WOS关键词RESERPINE
资助项目Science and Technology Commission of Shanghai Municipality[08DZ1980200]
WOS研究方向Biochemistry & Molecular Biology ; Chemistry
语种英语
WOS记录号WOS:000270246400040
出版者ELSEVIER SCIENCE BV
源URL[http://119.78.100.183/handle/2S10ELR8/279118]  
专题上海药物代谢研究中心
通讯作者Chen, Xiaoyan
作者单位1.Zhengzhou Univ, Zhengzhou 450001, Peoples R China
2.Chinese Acad Sci, Shanghai Inst Mat Med, Ctr Drug Metab & Pharmacokinet Res, Shanghai 201203, Peoples R China;
推荐引用方式
GB/T 7714		 Zhang, Huaicheng,Zhong, Dafang,Zhang, Zhenzhong,et al. Liquid chromatography/tandem mass spectrometry method for the quantification of deserpidine in human plasma: Application to a pharmacokinetic study[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2009,877(27):3221-3225.
APA Zhang, Huaicheng,Zhong, Dafang,Zhang, Zhenzhong,Dai, Xiaojian,&Chen, Xiaoyan.(2009).Liquid chromatography/tandem mass spectrometry method for the quantification of deserpidine in human plasma: Application to a pharmacokinetic study.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,877(27),3221-3225.
MLA Zhang, Huaicheng,et al."Liquid chromatography/tandem mass spectrometry method for the quantification of deserpidine in human plasma: Application to a pharmacokinetic study".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 877.27(2009):3221-3225.

入库方式： OAI收割

来源：上海药物研究所

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