Simultaneous determination of 6R-leucovorin, 6S-leucovorin and 5-methyltetrahydrofolate in human plasma using solid phase extraction and chiral liquid chromatography-tandem mass spectrometry
文献类型：期刊论文
| 作者 | Liu, Ke; Dai, Xiaojian ; Zhong, Dafang; Deng, Pan; Ma, Jinfei; Chen, Xiaoyan
|
| 刊名 | JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
 |
| 出版日期 | 2009-04-01 |
| 卷号 | 877期号:10页码:902-910 |
| 关键词 | Stereoselective separation Leucovorin 5-Methyltetrahydrofolate LC-MS/MS |
| ISSN号 | 1570-0232 |
| DOI | 10.1016/j.jchromb.2009.02.046 |
| 文献子类 | Article |
| 英文摘要 | A selective and rapid method was developed and validated for determination of 6R-leucovorin (LV), 6S-leucovorin and 5-methyltetrahydrofolate (5-MeTHF) in human plasma using stereoselective liquid chromatography-tandem mass spectrometry. All analytes and the internal standard were extracted from plasma by solid phase extraction using Oasis (R) HLB C-18 cartridges. A macrocyclic glycopeptide teicoplanin column was used for chiral separation of LV and 5-MeTHF isomers with NH(4)TFA or NH4OAc in methanol as mobile phase. Detection was performed on an API 4000 tandem mass spectrometer with positive electrospray ionization in multiple reaction monitoring mode. The calibration curves were linear in the range of 0.050-20.0 mu g/mL for 6R-LV and 6S-LV, and 0.025-10.0 mu g/mL for 5-MeTHE The intra- and inter-assay precision was 3.6-13.2%, 3.4-12.9% and 5.3-9.3% for 6R-LV, 6S-LV and 5-MeTHF, respectively. The accuracy was 99.4-102.4%, 95.3-96.8% and 93.0-110% for 6R-LV, 6S-LV and 5-MeTHF, respectively. The lower limit of quantification (LLOQ) was 0.050 mu g/mL for each LV isomer and 0.025 mu g/mL for 5-MeTHF. The method was successfully applied to a comparative pharmacokinetic study between leucovorin calcium and levoleucovorin calcium in 10 volunteers. No significant differences between levoleucovorin and leucovorin in pharmacokinetic parameters of 6S-LV and 5-MeTHF were found in volunteers. (C) 2009 Elsevier B.V. All rights reserved. |
| WOS关键词 | STATIONARY PHASES ; FOLINIC ACID ; HUMAN SERUM ; LEUCOVORIN ; FOLATE ; DIASTEREOISOMERS ; COLUMN ; PHARMACOKINETICS ; QUANTIFICATION ; SEPARATION |
| 资助项目 | National Basic Research Program of China[2009CB930300] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000265130100008 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/279280]  |
| 专题 | 上海药物代谢研究中心 |
| 通讯作者 | Chen, Xiaoyan |
| 作者单位 | Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Ke,Dai, Xiaojian,Zhong, Dafang,et al. Simultaneous determination of 6R-leucovorin, 6S-leucovorin and 5-methyltetrahydrofolate in human plasma using solid phase extraction and chiral liquid chromatography-tandem mass spectrometry[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2009,877(10):902-910. |
| APA | Liu, Ke,Dai, Xiaojian,Zhong, Dafang,Deng, Pan,Ma, Jinfei,&Chen, Xiaoyan.(2009).Simultaneous determination of 6R-leucovorin, 6S-leucovorin and 5-methyltetrahydrofolate in human plasma using solid phase extraction and chiral liquid chromatography-tandem mass spectrometry.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,877(10),902-910. |
| MLA | Liu, Ke,et al."Simultaneous determination of 6R-leucovorin, 6S-leucovorin and 5-methyltetrahydrofolate in human plasma using solid phase extraction and chiral liquid chromatography-tandem mass spectrometry".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 877.10(2009):902-910. |
入库方式： OAI收割
来源：上海药物研究所
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