Determination of eptifibatide concentration in human plasma utilizing the liquid chromatography-tandem mass spectrometry method
文献类型：期刊论文
| 作者 | Liu, Jia ; Duan, Xiaotao; Chen, Xiaoyan; Zhong, Dafang
|
| 刊名 | JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
 |
| 出版日期 | 2009-02-15 |
| 卷号 | 877期号:5-6页码:527-532 |
| 关键词 | Eptifibatide LC-MS/MS Plasma |
| ISSN号 | 1570-0232 |
| DOI | 10.1016/j.jchromb.2008.12.060 |
| 文献子类 | Article |
| 英文摘要 | A sensitive and selective liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed to determine the concentration of eptifibatide in human plasma. Following protein precipitation, the analyte was separated on a reversed-phase C-18 column. Acetonitrile:5 mM ammonium acerate:acetic acid (30:70:0.1, v/v/v) was used at a flow-rate of 0.5 mL/min with the isocratic mobile phase. An API 4000 tandem mass spectrometer equipped with a Turbo IonSpray ionization source was used as the detector and was operated in the positive ion mode. "Truncated" multiple reaction monitoring using the transition of m/z 832.6 -> m/z 832.6 and m/z 931.3 -> m/z 931.3 was performed to quantify eprifibatide and the internal standard (EPM-05), respectively. The method had a lower limit of quantification of 4.61 ng/mL for eptifibatide. The calibration Curve was demonstrated to be linear over the concentration range of 4.61 - 2770 ng/mL The intra- and inter-day precisions were less than 10.5% for each QC level, and the inter-day relative errors were 2.0%, 5.6%, and 2.8% for 9.22, 184, and 2490 ng/mL, respectively. The validated method was successfully applied to the quantification of eptifibatide concentration in human plasma after intravenous (i.v.) administration of a 270-mu g/kg bolus of eptifibatide and i.v. administration of eptifibatide at a constant rate of infusion of 2 mu g/(kg min) for 18 h in order to evaluate the pharmacokinetics. (C) 2009 Elsevier B.V. All rights reserved. |
| WOS关键词 | QUANTIFICATION ; MS/MS |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000263623900011 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/279312]  |
| 专题 | 上海药物代谢研究中心 |
| 通讯作者 | Zhong, Dafang |
| 作者单位 | Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Jia,Duan, Xiaotao,Chen, Xiaoyan,et al. Determination of eptifibatide concentration in human plasma utilizing the liquid chromatography-tandem mass spectrometry method[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2009,877(5-6):527-532. |
| APA | Liu, Jia,Duan, Xiaotao,Chen, Xiaoyan,&Zhong, Dafang.(2009).Determination of eptifibatide concentration in human plasma utilizing the liquid chromatography-tandem mass spectrometry method.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,877(5-6),527-532. |
| MLA | Liu, Jia,et al."Determination of eptifibatide concentration in human plasma utilizing the liquid chromatography-tandem mass spectrometry method".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 877.5-6(2009):527-532. |
入库方式： OAI收割
来源：上海药物研究所
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