Pharmacokinetics of nanoparticle-docetaxei injection in Beagle dogs
文献类型：期刊论文
| 作者 | Wu YW(武亦文)1; Wen J(闻俊)1; Fan GR(范国荣)1; Li YP(李亚平)2 ; Wu YT(吴玉田)1
|
| 刊名 | Chinese Journal of New Drugs and Clinical Remedies
 |
| 出版日期 | 2008 |
| 卷号 | 27期号:5页码:323-328 |
| 关键词 | docetaxel nanoparticles pharmacokinetics chromatography high pressure liquid spectrometry mass electrospray ionization |
| ISSN号 | 1007-7669 |
| 其他题名 | 注射用多西他赛纳米粒在犬体内的药动学研究 |
| 文献子类 | Article |
| 英文摘要 | AIM To develop a high-throughput analytical method based on LC-MS/MS for the determination of docetaxel and investigate the pharmacokinetics of the two docetaxel preparations in Beagle dog plasma. METHODS Docetaxel and paclitaxel (internal standard,IS) were isolated from 50muL plasma samples in 96- well plates by liquid-liquid extraction,and most liquid transfer steps were performed by eight-channel pipetting tools.The concentrations of docetaxel in plasma were determined by LC-MS/MS method and the pharmacokinetic parameters were calculated.RESULTS The calibration curves were linear over the range of 1-500mug·L~(-1).The lower limit of quantitation was 1mug·L~(-1).The intra-run and inter-run relative standard deviation (RSD) was less than 15%.The main pharmacokinetic parameters of nanoparticle-docetaxel injection and docetaxle normal injection were as follows:t_(1/2) were (5.3s 1.4) and (3.21.4) h,MRT were (3.21.3) and (0.660.21) h,V_(ss) were (8348) and (156) L,respectively.The differences of t_(1/2),MRT and V_(ss) were significant between two preparations.CONCLUSION The method is sensitive,accurate,convenient and high- throughput for the pharmacokinetic study of nanoparticle-docetaxel injections.Compared with docetaxel normal injection,nanoparticle-docetaxel injection has a controlled releasing rate,a high level of blood concentrations and a long blood circulation time,which are benefit for the control of acute toxicity and the improvement of tolerance to a certain extent. |
| WOS研究方向 | Pharmacology & Pharmacy (provided by Clarivate Analytics) |
| 语种 | 中文 |
| CSCD记录号 | CSCD:3280685 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/268045]  |
| 专题 | 药物制剂研究中心 |
| 作者单位 | 1.中国人民解放军第二军医大学药学院药物分析教研室, 上海 200433, 中国.; 2.中国科学院上海药物所, 上海 201203, 中国. |
| 推荐引用方式 GB/T 7714 | Wu YW,Wen J,Fan GR,et al. Pharmacokinetics of nanoparticle-docetaxei injection in Beagle dogs[J]. Chinese Journal of New Drugs and Clinical Remedies,2008,27(5):323-328. |
| APA | 武亦文,闻俊,范国荣,李亚平,&吴玉田.(2008).Pharmacokinetics of nanoparticle-docetaxei injection in Beagle dogs.Chinese Journal of New Drugs and Clinical Remedies,27(5),323-328. |
| MLA | 武亦文,et al."Pharmacokinetics of nanoparticle-docetaxei injection in Beagle dogs".Chinese Journal of New Drugs and Clinical Remedies 27.5(2008):323-328. |
入库方式： OAI收割
来源：上海药物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。