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Optimal extraction of Phaeoporus obliquus saponins and its scavenging effect on fat accumulation in HepG2 cells

文献类型：期刊论文

作者Zhang Lei1; Zhang Kun2; Wang Deli3; Ren Xiaohong4; Cui Na1; Liu Yang1; Zhao Tingxun1
刊名Journal of Jilin Univeristy. Medicine edition
出版日期2018
卷号44期号:3页码:526-531
关键词Phaeoporus obliquus saponins HepG2 cells triglyceride fat accumulation
ISSN号1671-587X
其他题名桦褐孔菌皂苷的优化提取及其对HepG2细胞脂肪堆积的清除作用
文献子类Article
英文摘要Objective: To investigate the ability of Phaeoporus obliquus saponins in scavenging the fat accumulation of liver cells,and to clarify its effect on the prevention of non-alcoholic fatty liver disease(NAFLD).Methods: The safe concentrations of Phaeoporus obliquus in the HepG2 cells was selected by MTT method. The cells treated with 60.000,6.000,0.600,0.060,and 0.006 g·L~(-1) Phaeoporus obliquus saponins were regarded as treatment groups; the blank well without drugs and cells was regarded as control group,and the HepG2 cells without drugs were regarded as model group.The survival rates of HepG2 cells in various groups were detected by MTT method;the clearance rates of triglyceride(TG) in the HepG2 cells in various groups were detected; the morphology of HepG2 cells was detected by oil red O staining. Results: The optimum extraction condition of Phaeoporus obliquus saponins were as followed:the liquid ratio was 1∶15,the extraction temperature was 30℃, the extraction time was 30 min, and the ultrasonic power was 50%.Compared with control group,the survival rate of HepG2 cells in 0.060 g·L~(-1) Phaeoporus obliquus saponins group was decreased and the clearance rate of TG was increased(P<0.05),and the lipid interfusion in cells was significantly reduced.Compared with control group,the survival rate of HepG2 cells in 0.600 g·L~(-1) Phaeoporus obliquus saponins group was decreased and the clearance rate of TG was increased(P<0.01),and the number of lipid drops was significantly reduced. Conclusion: The extraction rate of Phaeoporus obliquus saponins is obviously improved under the optimum extraction conditions and it has good in vitro lipid-lowering activity,and it has better preventive effect on NAFLD.
资助项目黑龙江省科技厅重点创新预研项目资助课题[00000000]
WOS研究方向General & Internal Medicine (provided by Clarivate Analytics)
语种中文
CSCD记录号CSCD:6268214
源URL[http://119.78.100.183/handle/2S10ELR8/269169]  
专题药物制剂研究中心
作者单位1.Department of Pharmaceutical Engineering, College of Life Science and Technology,Mudanjiang Normal University, Mudanjiang, Heilongjiang 157011, China.;
2.Research Center,Second Hospital, Jilin University, Changchun, Jilin 130041, China.;
3.Domonstration Center for Biological Experiments,School of Life Sciences,Jilin University, Changchun, Jilin 130012, China.;
4.Center of Pharmaceutical Preparations,Chinese Academy of Sciences, Shanghai Institute of Materia Medica, Shanghai 201210, China.
推荐引用方式
GB/T 7714		 Zhang Lei,Zhang Kun,Wang Deli,et al. Optimal extraction of Phaeoporus obliquus saponins and its scavenging effect on fat accumulation in HepG2 cells[J]. Journal of Jilin Univeristy. Medicine edition,2018,44(3):526-531.
APA Zhang Lei.,Zhang Kun.,Wang Deli.,Ren Xiaohong.,Cui Na.,...&Zhao Tingxun.(2018).Optimal extraction of Phaeoporus obliquus saponins and its scavenging effect on fat accumulation in HepG2 cells.Journal of Jilin Univeristy. Medicine edition,44(3),526-531.
MLA Zhang Lei,et al."Optimal extraction of Phaeoporus obliquus saponins and its scavenging effect on fat accumulation in HepG2 cells".Journal of Jilin Univeristy. Medicine edition 44.3(2018):526-531.

入库方式： OAI收割

来源：上海药物研究所

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