Photo-sensitive liposomes loading doxorubicin hydrochloride reverse drug resistance of breast cancer
文献类型：期刊论文
| 作者 | Meng Qingshuo; Zhang Pengcheng ; Yin Qi; Zhang Zhiwen; Yu Haijun; Li Yaping
|
| 刊名 | Acta Pharmaceutica Sinica
 |
| 出版日期 | 2017 |
| 卷号 | 52期号:5页码:809-820 |
| 关键词 | breast neoplasm doxorubicin drug resistance liposome photodynamic therapy photo-sensitive |
| ISSN号 | 0513-4870 |
| 其他题名 | 光敏感型盐酸多柔比星脂质体逆转乳腺癌耐药 |
| 文献子类 | Article |
| 英文摘要 | This study was aimed to build a new photo-sensitive co-delivery liposomes which combine photodynamic therapy with chemotherapy to reverse drug resistance of breast cancer. Photodynamic photosensitizer chlorin e6 trimethyl ester (Ce6tM) and chemotherapeutic drug doxorubicin hydrochloride (DOX) were loaded into the liposomes (liposomes loaded with Ce6tM and DOX, CDL) by thin-film hydration extrusion and ammonium sulfate active loading methods. CDL was characterized with cryo-transmission electron microscopy (Cryo-TEM), dynamic light scattering particle size, zeta potentials and photo-sensitive DOX release behaviors in vitro. CDL cytotoxicity, singlet oxygen production, DOX accumulation, intracellular ATP level and cell cycle analysis in MCF7/ADR cells were evaluated. Finally, the tissue distribution of DOX and antitumor effects of CDL in BALB/c-nu nude mice bearing MCF7/ADR tumor were investigated. The results showed that the particle size of obtained CDL was 90.7 1.1 nm and distributed uniformly. CDL possessed outstanding properties of photo-sensitive drug release profile. The accumulated release of DOX reached (96.52 0.11) % in 2 min under 671 nm laser irradiation (2 W·cm~(-2)). Interestingly, DOX in CDL could maintain rapid release after 671 nm laser irradiation with low power and short time (15 s, 0.25 W·cm~(-2)). This phenomenon was caused by oxidation of unsaturated phospholipids in CDL under 671 nm laser irradiation and had nothing to do with the slightly elevated temperature. Photo-sensitive drug release behavior contributed to increased DOX accumulation in MCF7/ADR cells. The half inhibition concentration (IC_(50)) of DOX in CDL laser group in MCF7/ADR cells was decreased by 601.9-fold compared with no laser group, which could be related to increased accumulation of DOX, decreased ATP levels and cell cycle arrest in MCF7/ADR cells. With the help of CDL, DOX accumulation in tumor was increased and in cardiac toxicity was reduced in vivo. CDL laser group showed a good anti-tumor effect. The tumor inhibition rate was (94.7 6.2) %. These results suggest that CDL has a promising potential in reversing drug resistance of breast cancer. |
| 资助项目 | 国家自然科学基金创新研究群体项目[00000000] |
| WOS研究方向 | Pharmacology & Pharmacy (provided by Clarivate Analytics) |
| 语种 | 中文 |
| CSCD记录号 | CSCD:5981477 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/269206]  |
| 专题 | 药物制剂研究中心 |
| 通讯作者 | Li Yaping |
| 作者单位 | Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China. |
| 推荐引用方式 GB/T 7714 | Meng Qingshuo,Zhang Pengcheng,Yin Qi,et al. Photo-sensitive liposomes loading doxorubicin hydrochloride reverse drug resistance of breast cancer[J]. Acta Pharmaceutica Sinica,2017,52(5):809-820. |
| APA | Meng Qingshuo,Zhang Pengcheng,Yin Qi,Zhang Zhiwen,Yu Haijun,&Li Yaping.(2017).Photo-sensitive liposomes loading doxorubicin hydrochloride reverse drug resistance of breast cancer.Acta Pharmaceutica Sinica,52(5),809-820. |
| MLA | Meng Qingshuo,et al."Photo-sensitive liposomes loading doxorubicin hydrochloride reverse drug resistance of breast cancer".Acta Pharmaceutica Sinica 52.5(2017):809-820. |
入库方式： OAI收割
来源：上海药物研究所
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