Constructed and Characterization of Tumor Microenvironment-sensitive Drug Delivery System
文献类型：期刊论文
| 作者 | Tan Jiao1; Chen Yingzhi2; Lin Tingting2; Liang Jianming2; Yang Yongxin2; Sun Xun1; Huang Yongzhuo2
|
| 刊名 | Chinese Journal of Modern Applied Pharmacy
 |
| 出版日期 | 2014 |
| 卷号 | 31期号:11页码:1297-1301 |
| 关键词 | trichosanthin PEGylation tumor microenvironment pH sensitive legumain |
| ISSN号 | 1007-7693 |
| 其他题名 | 肿瘤微环境双重响应性的智能型蛋白毒素给药系统的构建和表征 |
| 文献子类 | Article |
| 英文摘要 | OBJECTIVE To achieve tumor microenvironment-sensitive drug delivery which was prepared by recombinant and PEGylation techniques. METHODS A substrate peptide sequence of asparaginyl endopeptidase (legumain) was introduced to the C-terminus of trichosanthin(TCS) by recombinant technique; the recombinant plasmid was transformed into E. coli BL21(DE3) competent cells. TCS-Asn10-Cys recombinant protein was expressed and purified according a standard protocol. Further, the C-terminus thiol of the recombinant protein was conjugated to mPEG-Hz-Mal. The TCS-Asn10-Hz-PEG conjugate was purified using CM sepharose fast flow column. The pH-sensitivity and enzyme-sensitivity of TCS-Asn10-Hz-PEG in vitro were tested. RESULTS The TCS-Asn10-Cys fusion protein was successfully expressed and purified. The tumor microenvironment-drug delivery system of TCS-Asn10-Hz-PEG was prepared. In either the pH 5.6 medium or the presence of legumain, the PEG chain could be cleaved by hydrolysis or proteolysis from the TCS, of which the activity was recovered. CONCLUSION It is developed a dual-sensitive drug delivery system of TCS-Asn10-Hz-PEG that can be responsive to either acidic hydrolysis or legumain proteolysis. |
| 资助项目 | 国家自然科学基金[00000000] |
| WOS研究方向 | Pharmacology & Pharmacy (provided by Clarivate Analytics) |
| 语种 | 中文 |
| CSCD记录号 | CSCD:5302174 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/269305]  |
| 专题 | 药物制剂研究中心 |
| 通讯作者 | Huang Yongzhuo |
| 作者单位 | 1.West China School of Pharmacy, Sichuan University, Chengdu, Sichuan 610041, China.; 2.Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China. |
| 推荐引用方式 GB/T 7714 | Tan Jiao,Chen Yingzhi,Lin Tingting,et al. Constructed and Characterization of Tumor Microenvironment-sensitive Drug Delivery System[J]. Chinese Journal of Modern Applied Pharmacy,2014,31(11):1297-1301. |
| APA | Tan Jiao.,Chen Yingzhi.,Lin Tingting.,Liang Jianming.,Yang Yongxin.,...&Huang Yongzhuo.(2014).Constructed and Characterization of Tumor Microenvironment-sensitive Drug Delivery System.Chinese Journal of Modern Applied Pharmacy,31(11),1297-1301. |
| MLA | Tan Jiao,et al."Constructed and Characterization of Tumor Microenvironment-sensitive Drug Delivery System".Chinese Journal of Modern Applied Pharmacy 31.11(2014):1297-1301. |
入库方式： OAI收割
来源：上海药物研究所
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