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Cell-penetrating chimeric apoptotic peptide AVPI-LMWP/DNA co-delivery system for cancer therapy

文献类型：期刊论文

作者Tan Jiao2; Wang Yaping1; Wang Huixin1; Liang Jianming1; Zhang Meng1; Sun Xun2; Huang Yongzhuo1
刊名Acta Pharmaceutica Sinica
出版日期2014
卷号49期号:12页码:1718-1723
关键词TRAIL AVPI cell penetrating peptide apoptotic peptide gene delivery apoptosis TRAIL AVPI cancer therapy
ISSN号0513-4870
其他题名具有穿膜功能的嵌合型AVPI-低分子量鱼精蛋白/DNA共给药系统的抗肿瘤研究
文献子类Article
英文摘要To develop a cell-penetrating chimeric apoptotic peptide AVPI-LMWP/DNA co-delivery system for cancer therapy, we prepared the AVPI-LMWP/pTRAIL self-assembled complexes containing a therapeutic combination of peptide drug AVPI and DNA drug TRAIL. The chimeric apoptotic peptide AVPI-LMWP was synthesized using the standard solid-phase synthesis. The cationic AVPI-LMWP could condense pTRAIL by electrostatic interaction. The physical-chemical properties of the AVPI-LMWP/pTRAIL complexes were characterized. The cellular uptake efficiency and the inhibitory activity of the AVPI-LMWP/pTRAIL complexes on tumor cell were also performed. The results showed that the AVPI-LMWP/pTRAIL complexes were successfully prepared by co-incubation. With the increase of mass ratio (AVPI-LMWP/DNA), the particle size was decreased and the zeta potential had few change. Agarose gel electrophoresis showed that AVPI- LMWP could fully bind and condense pTRAIL at a mass ratio above 15∶1. Cellular uptake efficiency was improved along with the increased ratio of W_(AVPI-LMWP)/W_(pTRAIL). The in vitro cytotoxicity experiments demonstrated that the AVPI-LMWP/pTRAIL (W∶W = 20∶1) complexes was significantly more effective than the pTRAIL, AVPI-LMWP alone or LMWP/pTRAIL complexes on inhibition of HeLa cell growth. Our studies indicated that the AVPI-LMWP/pTRAIL co-delivery system could deliver plasmid into HeLa cell and induce tumor cell apoptosis efficiently, which showed its potential in cancer therapy using combination of apoptoic peptide and gene drugs.
资助项目国家自然科学基金资助项目[00000000]
WOS研究方向Pharmacology & Pharmacy (provided by Clarivate Analytics)
语种中文
CSCD记录号CSCD:5299740
源URL[http://119.78.100.183/handle/2S10ELR8/269307]  
专题药物制剂研究中心
通讯作者Huang Yongzhuo
作者单位1.Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China.
2.West China School of Pharmacy, Sichuan University, Chengdu, Sichuan 610041, China.;
推荐引用方式
GB/T 7714		 Tan Jiao,Wang Yaping,Wang Huixin,et al. Cell-penetrating chimeric apoptotic peptide AVPI-LMWP/DNA co-delivery system for cancer therapy[J]. Acta Pharmaceutica Sinica,2014,49(12):1718-1723.
APA Tan Jiao.,Wang Yaping.,Wang Huixin.,Liang Jianming.,Zhang Meng.,...&Huang Yongzhuo.(2014).Cell-penetrating chimeric apoptotic peptide AVPI-LMWP/DNA co-delivery system for cancer therapy.Acta Pharmaceutica Sinica,49(12),1718-1723.
MLA Tan Jiao,et al."Cell-penetrating chimeric apoptotic peptide AVPI-LMWP/DNA co-delivery system for cancer therapy".Acta Pharmaceutica Sinica 49.12(2014):1718-1723.

入库方式： OAI收割

来源：上海药物研究所

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