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A validated UPLC-MS/MS method coupled with protein precipitation and ion exchange solid phase extraction for the quantitation of porcine relaxin B29 in dog plasma and its application to a pharmacokinetic study

文献类型：期刊论文

作者Su, Chong2; Sun, Hui2; Yang, Hong2; Yin, Lei2; Zhang, Jiwen3; Fawcett, John Paul1; Gu, Jingkai2
刊名ANALYTICAL AND BIOANALYTICAL CHEMISTRY
出版日期2017-11
卷号409期号:28页码:6559-6565
关键词Ion exchange solid phase extraction LC-MS/MS Porcine relaxin Ultrahigh performance liquid chromatography
ISSN号1618-2642
DOI10.1007/s00216-017-0604-3
文献子类Article
英文摘要Porcine relaxin is a 6 kDa peptide hormone of pregnancy with important physiological and pharmacological effects. It contains a number of analogs of which porcine relaxin B29 is one of the most important. To support the development of porcine relaxin B29 as a new drug, we established an UPLC-MS/MS method for its quantitation in dog plasma. Sample preparation by protein precipitation and ion exchange solid phase extraction was followed by UPLC on an XBridge (TM) BEH300 C18 column at 40 A degrees C in a run time of only 5.5 min. Detection was performed on a Qtrap 6500 mass spectrometer using ESI in the positive ion mode with MRM of the transitions at m/z 831.7 [M+7H](7+) -> 505.4 and m/z 1162.4 [M+5H](5+) -> 226 for pRLX B29 and internal standard (recombinant human insulin), respectively. The method was linear over the concentration range 30-2000 ng/mL with no matrix effects. Intra- and inter-day precisions were < 15% with accuracies in the range 98.8-100.6%. The method was successfully applied to a pharmacokinetic study in beagle dogs after administration of a 0.15 mg/kg intravenous dose.
WOS关键词CHROMATOGRAPHY-MASS SPECTROMETRY ; RECOMBINANT HUMAN RELAXIN-2 ; IMMUNOAFFINITY PURIFICATION ; HIGH RESOLUTION ; INSULIN ; QUANTIFICATION ; PEPTIDES
资助项目National Natural Science Foundation of China[81430087] ; National Natural Science Foundation of China[81473142] ; National Natural Science Foundation of China[81673396] ; National Natural Science Foundation of China[81673502] ; National Natural Science Foundation of China[81603182]
WOS研究方向Biochemistry & Molecular Biology ; Chemistry
语种英语
WOS记录号WOS:000414329900004
出版者SPRINGER HEIDELBERG
源URL[http://119.78.100.183/handle/2S10ELR8/272426]  
专题药物制剂研究中心
通讯作者Gu, Jingkai
作者单位1.Univ Otago, Sch Pharm, POB 56, Dunedin 9054, New Zealand
2.Jilin Univ, Sch Life Sci, 2699 Qinajin Rd, Changchun 130012, Jilin, Peoples R China;
3.Chinese Acad Sci, Shanghai Inst Mat Med, Ctr Drug Delivery Syst, Shanghai 201203, Peoples R China;
推荐引用方式
GB/T 7714		 Su, Chong,Sun, Hui,Yang, Hong,et al. A validated UPLC-MS/MS method coupled with protein precipitation and ion exchange solid phase extraction for the quantitation of porcine relaxin B29 in dog plasma and its application to a pharmacokinetic study[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2017,409(28):6559-6565.
APA Su, Chong.,Sun, Hui.,Yang, Hong.,Yin, Lei.,Zhang, Jiwen.,...&Gu, Jingkai.(2017).A validated UPLC-MS/MS method coupled with protein precipitation and ion exchange solid phase extraction for the quantitation of porcine relaxin B29 in dog plasma and its application to a pharmacokinetic study.ANALYTICAL AND BIOANALYTICAL CHEMISTRY,409(28),6559-6565.
MLA Su, Chong,et al."A validated UPLC-MS/MS method coupled with protein precipitation and ion exchange solid phase extraction for the quantitation of porcine relaxin B29 in dog plasma and its application to a pharmacokinetic study".ANALYTICAL AND BIOANALYTICAL CHEMISTRY 409.28(2017):6559-6565.

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来源：上海药物研究所

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