Simultaneous determination of eight B-vitamins in rat intestinal perfusate to identify effects of osmotic pressures on absorptions
文献类型：期刊论文
| 作者 | Wang, Xiaobo2; Xiao, Jian3; Zhou, Hui2; Qiu, Ying2; Peng, Hui3; Sun, Yangyang2; Zhang, Jiwen1,2 ; Qian, Wei3; Sun, Lixin2
|
| 刊名 | BIOMEDICAL CHROMATOGRAPHY
 |
| 出版日期 | 2017-09 |
| 卷号 | 31期号:9 |
| 关键词 | B-vitamins HPLC-DAD intestinal perfusate osmotic pressures |
| ISSN号 | 0269-3879 |
| DOI | 10.1002/bmc.3952 |
| 文献子类 | Article |
| 英文摘要 | A rapid and accurate HPLC-DAD method was developed and validated to simultaneously determine eight B-vitamins (VBs, namely thiamine, riboflavin, niacinamide, calcium pantothenic, pyridoxine, biotin, folic acid and cyanocobalamin) and phenolsulfonphthalein in rat intestinal perfusate. Chromatographic separation was achieved using an Inertsil ODS-3 column (250 x 4.6 mm i.d., 5 mu m) at a temperature of 40 degrees C. Gradient elution mode was applied at the flow rate of 1.0 mL/min with the mobile phase of acetonitrile-30 mm K2HPO4 (pH5.80). The method was successfully applied to identify the effects of osmotic pressures on the absorption of the VBs. The absorption profiles of single and mixed VBs were also compared. Histological section technology was applied to observe the microstructure of small bowel mucosa after perfusion. The results indicated that each compound possessed a better absorption profile under isotonic conditions than under hypotonic or hypertonic conditions for single or mixed solutions. Compared with single VBs, better absorptions in mixed VBs were observed. Pathological tissue slice test suggested that hypotonic and hypertonic solutions changed or damaged the microstructure of mucosa to varying degrees. Taken together, the investigations indicated that multi-VBs administered orally under isotonic condition could generate fast and complete absorption profiles for VBs. |
| WOS关键词 | PERFORMANCE LIQUID-CHROMATOGRAPHY ; INFANT-FORMULA-COUNCIL ; WATER-SOLUBLE VITAMINS ; QUININE SOLUTIONS ; PANTOTHENIC-ACID ; DRUG ABSORPTION ; IN-VITRO ; TRANSPORT ; RIBOFLAVIN ; FOOD |
| 资助项目 | Project of Liaoning Distinguished Professor[00000000] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:000407272300009 |
| 出版者 | WILEY |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/272517]  |
| 专题 | 药物制剂研究中心 |
| 通讯作者 | Sun, Lixin |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai, Peoples R China 2.Shenyang Pharmaceut Univ, Sch Pharm, 103 Wenhua Rd, Shenyang 110016, Peoples R China; 3.By Hlth Co Ltd, Guangzhou, Guangdong, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Wang, Xiaobo,Xiao, Jian,Zhou, Hui,et al. Simultaneous determination of eight B-vitamins in rat intestinal perfusate to identify effects of osmotic pressures on absorptions[J]. BIOMEDICAL CHROMATOGRAPHY,2017,31(9). |
| APA | Wang, Xiaobo.,Xiao, Jian.,Zhou, Hui.,Qiu, Ying.,Peng, Hui.,...&Sun, Lixin.(2017).Simultaneous determination of eight B-vitamins in rat intestinal perfusate to identify effects of osmotic pressures on absorptions.BIOMEDICAL CHROMATOGRAPHY,31(9). |
| MLA | Wang, Xiaobo,et al."Simultaneous determination of eight B-vitamins in rat intestinal perfusate to identify effects of osmotic pressures on absorptions".BIOMEDICAL CHROMATOGRAPHY 31.9(2017). |
入库方式： OAI收割
来源：上海药物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。