Arginine-chitosan/DNA self-assemble nanoparticles for gene delivery: In vitro characteristics and transfection efficiency
文献类型：期刊论文
| 作者 | Gao, Yu2; Xu, Zhenghong2; Chen, Shangwei1; Gu, Wangwen2 ; Chen, Lingli2; Li, Yaping2
|
| 刊名 | INTERNATIONAL JOURNAL OF PHARMACEUTICS
 |
| 出版日期 | 2008-07-09 |
| 卷号 | 359期号:1-2页码:241-246 |
| 关键词 | self-assemble nanoparticles arginine-chitosan non-viral vector DNA |
| ISSN号 | 0378-5173 |
| DOI | 10.1016/j.ijpharm.2008.03.037 |
| 文献子类 | Article |
| 英文摘要 | Chitosan (Cs) is a natural cationic polysaccharide that has shown potential as non-viral vector for gene delivery because of its biocompatibility and low toxicity. However, chitosan used for gene delivery is limited due to its poorwater solubility and low transfection efficiency. The purpose of this work was to prepare Arginine-chitosan (Arg-Cs)/DNA self-assemble nanoparticles (ACSNs), and determine their in vitro characteristics and transfection efficiency against HEK 293 and COS-7 cells. Our experimental results showed that the particle size and zeta potential of ACSNs prepared with different N/P ratios were 200-400 nm and 0.23-12.25 mV, respectively. The in vitro transfection efficiency of ACSNs showed dependence on pH of transfection medium, and the highest expression efficiency was obtained at pH 7.2. The transfection efficiency increased with the ratio of chitosan-amine/DNA phosphate (N/P ratio) from 1 to 5, and reached the highest level with the N/P ratio 5. Effect of plasmid dosage on the transfection efficiency showed the highest transfection efficiency was obtained at 4 mu g/well for HEK 293 cells and 6 wg/well for COS-7 cells. The transfection efficiency of ACSNs was much higher than that of Cs/DNA self-assemble nanoparticles (CSNs). The average cell viability of ACSNs was over 90%. These results suggested that ACSNs could be a safe and effective non-viral vector for gene delivery. (C) 2008 Elsevier B.V. All rights reserved. |
| WOS关键词 | ACID-MODIFIED CHITOSAN ; DNA ; PEPTIDES ; CARRIER ; STRATEGIES ; VECTORS ; SYSTEMS ; SERUM |
| WOS研究方向 | Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:000257530400031 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/272867]  |
| 专题 | 药物制剂研究中心 上海中药现代化研究中心 |
| 通讯作者 | Li, Yaping |
| 作者单位 | 1.S Yangtze Univ, Testing & Anal Ctr, Wuxi 214122, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Mat Med, Ctr Drug Delivery Syst, Shanghai 201203, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Gao, Yu,Xu, Zhenghong,Chen, Shangwei,et al. Arginine-chitosan/DNA self-assemble nanoparticles for gene delivery: In vitro characteristics and transfection efficiency[J]. INTERNATIONAL JOURNAL OF PHARMACEUTICS,2008,359(1-2):241-246. |
| APA | Gao, Yu,Xu, Zhenghong,Chen, Shangwei,Gu, Wangwen,Chen, Lingli,&Li, Yaping.(2008).Arginine-chitosan/DNA self-assemble nanoparticles for gene delivery: In vitro characteristics and transfection efficiency.INTERNATIONAL JOURNAL OF PHARMACEUTICS,359(1-2),241-246. |
| MLA | Gao, Yu,et al."Arginine-chitosan/DNA self-assemble nanoparticles for gene delivery: In vitro characteristics and transfection efficiency".INTERNATIONAL JOURNAL OF PHARMACEUTICS 359.1-2(2008):241-246. |
入库方式： OAI收割
来源：上海药物研究所
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