Poly(imidazole/DMAEA)phosphazene/DNA self-assembled nanoparticles for gene delivery: Synthesis and in vitro transfection
文献类型：期刊论文
| 作者 | Yang, Yongxin1,2; Xu, Zhenghong1; Jiang, Jingui3; Gao, Yu1; Gu, Wangwen1 ; Chen, Lingli1; Tang, Xiaozheng3; Li, Yaping1
|
| 刊名 | JOURNAL OF CONTROLLED RELEASE
 |
| 出版日期 | 2008-05-08 |
| 卷号 | 127期号:3页码:273-279 |
| 关键词 | poly(imidazole/DMAEA)phosphazene nanoparticles non-viral vector gene delivery system |
| ISSN号 | 0168-3659 |
| DOI | 10.1016/j.jconrel.2008.01.012 |
| 文献子类 | Article |
| 英文摘要 | A new cationic derivate of polyphosphazene with imidazole and 2-dimethylaminoethylamino (DMAEA) as side groups, poly(imidazole/ DMAEA)phosphazene (PIDP), was synthesized and investigated for gene delivery. The half-lives of PIDP degradation under neutral (pH 7.4) and acidic conditions (pH 5.0) were 22 and 3 days at 37 degrees C, respectively. The cytotoxicity of PIDP assayed by MTT was much lower than that of poly (2-dimethylaminoethylamino)phosphazene (PDAP) and PEI 25K. PIDP could condense DNA into nanoparticles with a size around 100 nm and zeta potential (+25 mV) at the ratio of 10:1 (PIDP/DNA, w/w). The transfection efficiency of PIDP/DNA complex nanoparticles (PICNs) against 293T, COS-7 and Hela cells was much higher than that of PDAP/DNA complexes nanoparticles (PDCNs) and PEI/DNA complexes nanoparticles (PECNs) at 10:1 (polymer/DNA, w/w). Therefore, PIDP could be a safe, efficient and promising cationic polymer for gene therapy. (C) 2008 Elsevier B.V. All rights reserved. |
| WOS关键词 | CATIONIC POLYMERS ; MOLECULAR-WEIGHT ; DNA ; SYSTEMS ; VECTOR ; CELLS ; POLYETHYLENIMINE ; POLYPHOSPHAZENE ; ENDOCYTOSIS ; POLYPLEXES |
| WOS研究方向 | Chemistry ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:000256271800009 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/272919]  |
| 专题 | 药物制剂研究中心 |
| 通讯作者 | Li, Yaping |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, Ctr Drug Delivery Syst, Shanghai 201203, Peoples R China; 2.Fudan Univ, Sch Pharm, Dept Pharmaceut, Shanghai 200032, Peoples R China; 3.Shanghai Jiao Tong Univ, Sch Chem & Chem Engn, Shanghai 200240, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yang, Yongxin,Xu, Zhenghong,Jiang, Jingui,et al. Poly(imidazole/DMAEA)phosphazene/DNA self-assembled nanoparticles for gene delivery: Synthesis and in vitro transfection[J]. JOURNAL OF CONTROLLED RELEASE,2008,127(3):273-279. |
| APA | Yang, Yongxin.,Xu, Zhenghong.,Jiang, Jingui.,Gao, Yu.,Gu, Wangwen.,...&Li, Yaping.(2008).Poly(imidazole/DMAEA)phosphazene/DNA self-assembled nanoparticles for gene delivery: Synthesis and in vitro transfection.JOURNAL OF CONTROLLED RELEASE,127(3),273-279. |
| MLA | Yang, Yongxin,et al."Poly(imidazole/DMAEA)phosphazene/DNA self-assembled nanoparticles for gene delivery: Synthesis and in vitro transfection".JOURNAL OF CONTROLLED RELEASE 127.3(2008):273-279. |
入库方式： OAI收割
来源：上海药物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。