A multifunctional nano device as non-viral vector for gene delivery: In vitro characteristics and transfection
文献类型：期刊论文
| 作者 | Gao, Yu; Gu, Wangwen ; Chen, Lingli; Xu, Zhenghong; Li, Yaping
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| 刊名 | JOURNAL OF CONTROLLED RELEASE
 |
| 出版日期 | 2007-04-23 |
| 卷号 | 118期号:3页码:381-388 |
| 关键词 | non-viral vector pDNA multifunctional nano device folate protamine poly(cyanoacrylate) |
| ISSN号 | 0168-3659 |
| DOI | 10.1016/j.jconrel.2007.01.006 |
| 文献子类 | Article |
| 英文摘要 | The aim of this work was to design a new multifunctional nano device (MND) for gene delivery. This MND was equipped with folic acid as ligand, which was conjugated to terminal amido of poly(aminopoly(ethylene glycol)cyanoacrylate-co-hexadecyl cyanoacrylate) (poly (H(2)NPEGCA-co-HDCA)) to synthesize poly(Folate-HNPEGCA-co-HDCA), prolamine sulfate (PS) as DNA condenser and for nuclear transfer, PEG chain from poly(Folate-HNPEGCA-co-HDCA) for decreasing macrophages recognition and extending half-life, dioleoyl phosphatidylethanolamine (DOPE) for endosomal escape, and we supposed that the latent DOPE fusogenicity could be gently restored along with fast degradation of poly(Folate-HNPEGCA-co-HDCA) in MND membrane within endosome. Our experimental results showed that optimum complexation (similar to 97%) of DNA was achieved at DNA:PS = 1:3 (w/w). The MND showed different loading ratio by lipid film hydration technique with the highest loading ratio about 12%, the particle size range 200-400 nm, surface charge range 8 mV-15 mV. MND1 (poly(Folate-HNPEGCA-co-HDCA)/DOPE, 5:95, molar ratio) exhibited a high burst release effect with 60% of pDNA/PS released within 1 day at PBS (pH4.5), but with 21.4% and 8.1% pDNA/PS release at PBS with pH 5.8 and 7.4 within 24 It, respectively. However, lesser pDNA/PS release occurred in MND2 (poly(Folate-HNPEGCA-co-HDCA)[DOPE, 10:90, molar ratio) with 46%, 16.9% and 7.8% of pDNA/PS released at PBS with pH 4.5, 5.8 and 7.4 within 24 h, respectively. After I day, pDNA/PS displayed a sustained release pattern. The amount of cumulated pDNA/PS release over 3 days was 75% and 51.2% at PBS with pH 4.5 for MND1 and MND2, respectively. The MD loading pDNA/PS showed that luciferase activity was over 0.5 ng luciferase/mg protein in KB cells, in particular, the MND1 showed the highest transfection efficiency (0.66 ng luciferase/mg protein) in KB cells, which was much higher compared with in A549 cells or other formulations such as LipofectAMINE (TM) free pDNA/PS and control multifunctional nano device (CMND), whose lipid film was consisted of poly(H2NPEGCA-co-HDCA) and DOPE. In addition, MND also showed good protection during encapsulation and low cytotoxicity. As a result, MND could be a more potential non-viral vector for delivery of DNA. (c) 2007 Elsevier B.V. All rights reserved. |
| WOS关键词 | TARGETED DRUG-DELIVERY ; FOLATE RECEPTOR ; PLASMID DNA ; SENSITIVE LIPOSOMES ; CATIONIC LIPOSOMES ; FOLIC-ACID ; PROTAMINE ; NANOPARTICLES ; POLYETHYLENIMINE ; MICROSPHERES |
| WOS研究方向 | Chemistry ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:000246083900014 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/273282]  |
| 专题 | 药物制剂研究中心 上海中药现代化研究中心 |
| 通讯作者 | Li, Yaping |
| 作者单位 | Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Mat Med, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Gao, Yu,Gu, Wangwen,Chen, Lingli,et al. A multifunctional nano device as non-viral vector for gene delivery: In vitro characteristics and transfection[J]. JOURNAL OF CONTROLLED RELEASE,2007,118(3):381-388. |
| APA | Gao, Yu,Gu, Wangwen,Chen, Lingli,Xu, Zhenghong,&Li, Yaping.(2007).A multifunctional nano device as non-viral vector for gene delivery: In vitro characteristics and transfection.JOURNAL OF CONTROLLED RELEASE,118(3),381-388. |
| MLA | Gao, Yu,et al."A multifunctional nano device as non-viral vector for gene delivery: In vitro characteristics and transfection".JOURNAL OF CONTROLLED RELEASE 118.3(2007):381-388. |
入库方式： OAI收割
来源：上海药物研究所
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