Stability and release characteristics of poly(D,L-lactide-co-glycolide) encapsulated CaPi-DNA coprecipitation
文献类型：期刊论文
| 作者 | Li, YP ; Ogris, M; Pelisek, J; Roedl, W
|
| 刊名 | INTERNATIONAL JOURNAL OF PHARMACEUTICS
 |
| 出版日期 | 2004-01-09 |
| 卷号 | 269期号:1页码:61-70 |
| 关键词 | pDNA calcium phosphate coprecipitation stability microparticles poly(lactide-co-glycolide) |
| ISSN号 | 0378-5173 |
| DOI | 10.1016/j.ijpharm.2003.08.019 |
| 文献子类 | Article |
| 英文摘要 | The aims of this work were to determine the stability of DNA-calcium-phosphate coprecipitation (CaPi-DNA) against various conditions during double emulsification microencapsulation and assess the release and physicochemical characteristics of poly(D,L-lactide-co-glycolide) (PLGA) microparticles loading CaPi-DNA. CaPi-DNA prepared at pH 6.5 showed a good stability with over 60% CaPi-DNA remained after emulsification, but no more than 40% at pH 8.0. Polyvinyl alcohol (PVA, 1-5%) could make over 80% CaPi-DNA (pH 7.0) preserved after homogenization. The dichloromethane (DCM), mixture of DCM and ethyl acetate, ether and n-hexane (1:1) exhibited neglectable influence on CaPi-DNA under homogenization. PLGA had influenced on CaPi-DNA without any additional stabilizer, in particular, PLGA (75:25, 4%, w/v) demonstrated a profound damage with only about 10% of the original CaPi-DNA remained. PLGA microparticles loading CaPi-DNA were spherical in shape with size range of 2.0-5.0 mum, and entrapment efficiency 30-50%. CaPi-DNA was found to increase the stability of pDNA in PLGA microparticles without losing its structure integrity. The release of CaPi-DNA from microparticles showed a low burst release (<7.5%) within 24 h and following sustained release process. The amount of cumulated CaPi-DNA release over 30 days was: 17.6% for PLGA (lactide:glycolide = 50:50), 27.3% for PLGA (65:35) and 44.8% for PLGA (75:25) microparticles, respectively. The encapsulation of CaPi-DNA in microparticles could significantly protect CaPi-DNA from degradation of nuclease with average over 80% of total DNA recovery. These results suggested that the encapsulation of CaPi-DNA in PLGA microparticles could improve stability of pDNA. (C) 2003 Elsevier B.V. All rights reserved. |
| WOS关键词 | COMPLEXED PLASMID DNA ; GENE DELIVERY ; POLY(D,L-LACTIC-CO-GLYCOLIC ACID) ; CALCIUM-PHOSPHATE ; MICROSPHERES ; TRANSFECTION ; LIPOSOMES ; CELLS ; POLY(DL-LACTIDE-CO-GLYCOLIDE) ; MICROPARTICLES |
| WOS研究方向 | Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:000188509200007 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/274120]  |
| 专题 | 药物制剂研究中心 |
| 通讯作者 | Li, YP |
| 作者单位 | 1.Univ Munich, Dept Pharm, Ctr Drug Res, D-81377 Munich, Germany 2.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Mat Med, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, YP,Ogris, M,Pelisek, J,et al. Stability and release characteristics of poly(D,L-lactide-co-glycolide) encapsulated CaPi-DNA coprecipitation[J]. INTERNATIONAL JOURNAL OF PHARMACEUTICS,2004,269(1):61-70. |
| APA | Li, YP,Ogris, M,Pelisek, J,&Roedl, W.(2004).Stability and release characteristics of poly(D,L-lactide-co-glycolide) encapsulated CaPi-DNA coprecipitation.INTERNATIONAL JOURNAL OF PHARMACEUTICS,269(1),61-70. |
| MLA | Li, YP,et al."Stability and release characteristics of poly(D,L-lactide-co-glycolide) encapsulated CaPi-DNA coprecipitation".INTERNATIONAL JOURNAL OF PHARMACEUTICS 269.1(2004):61-70. |
入库方式： OAI收割
来源：上海药物研究所
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