Evidence for mu opioid receptor on mouse spleen lymphocytes
文献类型：期刊论文
| 作者 | Wu, T; Li, XY |
| 刊名 | ACTA PHARMACOLOGICA SINICA
 |
| 出版日期 | 1999-09 |
| 卷号 | 20期号:9页码:835-838 |
| 关键词 | fentanyl naloxone mu opioid receptors T-lymphocytes B-lymphocytes radioligand assay concanavalin A lipopolysaccharides thymidine spleen |
| ISSN号 | 0253-9756 |
| 文献子类 | Article |
| 英文摘要 | AIM: Using ohmefentanyl (Ohm), a high affinity Ct opioid receptor agonist, to find whether CL opioid receptor exists on mouse spleen lymphocytes. METHODS: The proliferation rates of T-lymphocytes and B-lymphocytes were determined under various concentrations of Ohm with or without naloxone(Nal) in vitro. Binding characteristics of [H-3]Ohm with mice spleen lymphocytes were studied by radioligand assays. RESULTS: Ohm 0.1 pmol L-1 -1 nmol L-1 enhanced Con A-induced spleen T-cell proliferation in vitro. Nal 50 mu mol.L-1, which per se enhanced the T-cell proliferation, blocked the enhancing effects of Ohm. However, Ohm had no effect on B-cell proliferation. Furthermore, a satisfactory saturable, specific, and reversible binding was demonstrated with Kd Of (6.9 +/- 0.6) nmol L-1 B-max of (74 +/- 6) fmol/10(7) cells. The binding of [3H]Ohm was blocked by unlabeled Ohm or Nal. CONCLUSION: Stimulating effects of Ohm on lymphocytes were mediated by opioid receptors. Mouse spleen lymphocytes present CL opioid receptors. |
| WOS关键词 | METHIONINE-ENKEPHALIN ; IMMUNE CELLS ; MORPHINE ; PROLIFERATION ; INVERTEBRATE |
| WOS研究方向 | Chemistry ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:000082602900011 |
| 出版者 | ACTA PHARMACOLOGICA SINICA |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/274702]  |
| 专题 | 药物制剂研究中心 |
| 通讯作者 | Li, XY |
| 作者单位 | Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 200031, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wu, T,Li, XY. Evidence for mu opioid receptor on mouse spleen lymphocytes[J]. ACTA PHARMACOLOGICA SINICA,1999,20(9):835-838. |
| APA | Wu, T,&Li, XY.(1999).Evidence for mu opioid receptor on mouse spleen lymphocytes.ACTA PHARMACOLOGICA SINICA,20(9),835-838. |
| MLA | Wu, T,et al."Evidence for mu opioid receptor on mouse spleen lymphocytes".ACTA PHARMACOLOGICA SINICA 20.9(1999):835-838. |
入库方式： OAI收割
来源：上海药物研究所
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