Poly-gamma-glutamic acid-based GGT-targeting and surface camouflage strategy for improving cervical cancer gene therapy
文献类型：期刊论文
| 作者 | Tan, Jiao2,4,5; Wang, Huiyuan5 ; Xu, Fan3,5; Chen, Yingzhi5; Zhang, Meng5; Peng, Huige5; Sun, Xun4; Shen, Youqing1; Huang, Yongzhuo5
|
| 刊名 | JOURNAL OF MATERIALS CHEMISTRY B
 |
| 出版日期 | 2017-02-14 |
| 卷号 | 5期号:6页码:1315-1327 |
| ISSN号 | 2050-750X |
| DOI | 10.1039/c6tb02990f |
| 文献子类 | Article |
| 英文摘要 | Polycation-based delivery presents a major method for non-viral gene therapy. However, the disadvantages of cationic vectors are their tendencies to be captured and eliminated by the reticuloendothelial system due to their excessive positive charges and nonspecific interaction with normal cells that leads to adverse effects. PEGylation was applied to solve these major problems. Yet, PEG chains can severely compromise cellular uptake and yield unsatisfying efficiency resulting in a so-called PEG dilemma. We developed a gamma-PGA-based GGT-targeting and surface camouflage strategy by constructing a ternary complex system via a layer-by-layer self-assembly method. The biodegradable polyanion gamma-PGA could protect the PEI/pDNA complexes from interaction with the body fluid components; however, in endosome, the polyanion facilitated the intracellular release of PEI/pDNA. The gamma-PGA/PEI/pDNA nanoparticles possessed a markedly improved serum-tolerant capability. More importantly, gamma-PGA interacts with the tumor-associated g-glutamyl transpeptidase (GGT) that can mediate endocytosis of the nanoparticles. With pTRAIL as the therapeutic gene, the gamma-PGA/PEI/pTRAIL nanoparticles effectively inhibited tumor cell proliferation by inducing cell apoptosis and arresting cell cycles. The in vivo results displayed effective suppression of tumor growth, and high treatment efficacy in the mice bearing cervical tumor. The gamma-PGA-based GGT-targeting and surface camouflage strategy is a potential method for improved gene delivery and cancer therapy. |
| WOS关键词 | HYALURONIC-ACID ; DELIVERY ; POLYETHYLENIMINE ; CELLS ; NANOPARTICLES ; COMPLEXES ; TUMORS ; TRANSFECTION ; EXPRESSION ; DILEMMA |
| 资助项目 | 973 Program, China[2014CB931900] ; 973 Program, China[2013CB932503] ; NFSC[81373357] ; NFSC[81422048] ; NFSC[81402883] ; NFSC[81521005] ; NFSC[81673382] ; Scientific Research and Equipment Development Project[YZ201437] ; Youth Innovation Promotion Association[00000000] |
| WOS研究方向 | Materials Science |
| 语种 | 英语 |
| WOS记录号 | WOS:000395914000020 |
| 出版者 | ROYAL SOC CHEMISTRY |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/275631]  |
| 专题 | 药物制剂研究中心 |
| 通讯作者 | Sun, Xun; Huang, Yongzhuo |
| 作者单位 | 1.Zhejiang Univ, Coll Chem & Biol Engn, Hangzhou, Zhejiang, Peoples R China 2.Third Mil Med Univ, Southwest Hosp, Chongqing, Peoples R China; 3.Univ Sci & Technol China, Nano Sci Tech Inst, Hefei, Peoples R China; 4.Sichuan Univ, West China Sch Pharm, Chengdu, Peoples R China; 5.Chinese Acad Sci, Shanghai Inst Mat Med, Beijing, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Tan, Jiao,Wang, Huiyuan,Xu, Fan,et al. Poly-gamma-glutamic acid-based GGT-targeting and surface camouflage strategy for improving cervical cancer gene therapy[J]. JOURNAL OF MATERIALS CHEMISTRY B,2017,5(6):1315-1327. |
| APA | Tan, Jiao.,Wang, Huiyuan.,Xu, Fan.,Chen, Yingzhi.,Zhang, Meng.,...&Huang, Yongzhuo.(2017).Poly-gamma-glutamic acid-based GGT-targeting and surface camouflage strategy for improving cervical cancer gene therapy.JOURNAL OF MATERIALS CHEMISTRY B,5(6),1315-1327. |
| MLA | Tan, Jiao,et al."Poly-gamma-glutamic acid-based GGT-targeting and surface camouflage strategy for improving cervical cancer gene therapy".JOURNAL OF MATERIALS CHEMISTRY B 5.6(2017):1315-1327. |
入库方式： OAI收割
来源：上海药物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。