中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
首页 机构 成果 学者
  
  1. CAS IR Grid
  2. 上海药物研究所
  3. 中国科学院上海药物研究所
  4. 分子影像中心（筹）
Peptide-Tethered Hydrogel Scaffold Promotes Recovery from Spinal Cord Transection via Synergism with Mesenchymal Stem Cells

文献类型：期刊论文

作者Li, Li-Ming9; Han, Min9; Jiang, Xin-Chi9; Yin, Xian-Zhen4; Chen, Fu8; Zhang, Tian-Yuan9; Ren, Hao7; Zhang, Ji-Wen4; Hou, Ting-Jun8; Chen, Zhong6
刊名ACS APPLIED MATERIALS & INTERFACES
出版日期2017-02-01
卷号9期号:4页码:3330-3342
关键词hyaluronic acid hydrogel scaffold mesenchymal stem cells adhesive peptide spinal cord injury tissue engineering
ISSN号1944-8244
DOI10.1021/acsami.6b12829
文献子类Article
英文摘要Spinal cord injury (SCI) is one of the most devastating injuries. Treatment strategies for SCI are required to overcome comprehensive issues. Implantation of biomaterial scaffolds and stem cells has been demonstrated to be a promising strategy. However, a comprehensive recovery effect is difficult to achieve. In the comprehensive treatment process, the specific roles of the implanted scaffolds and of stem cells in combined strategy are usually neglected. In this study, a peptide modified scaffold is developed based on hyaluronic acid and an adhesive peptide PPFLMLLKGSTR. Synchrotron radiation micro computed tomography measurement provides insights to the three-dimensional inner topographical property and perspective porous structure of the scaffold. The modified scaffold significantly improves cellular survival and adhesive growth of mesenchymal stem cells during 3D culture in vitro. After implantation in transected spinal cord, the modified scaffold and mesenchymal stems are found to function in synergy to restore injured spinal cord tissue, with respective strengths. Hindlimb motor function scores exhibit the most significant impact of the composite implant at 2 weeks post injury, which is the time secondary injury factors begin to take hold. Investigation on the secondary injury factors including inflammatory response and astrocyte overactivity at 10 days post injury reveals the possible underlying reason. Implants of the scaffold, cells, and especially the combination of both elicit inhibitory effects on these adverse factors. The study develops a promising implant for spinal cord tissue engineering and reveals the roles of the scaffold and stem cells. More importantly, the results provide the first understanding of the bioactive peptide PPFLMLLKGSTR concerning its functions on mesenchymal stem cells and spinal cord tissue restoration.
WOS关键词HYALURONIC-ACID HYDROGELS ; MARROW STROMAL CELLS ; FUNCTIONAL RECOVERY ; AXON REGENERATION ; GENE DELIVERY ; IN-VIVO ; INJURY ; REPAIR ; BRAIN ; TRANSPLANTATION
资助项目National Natural Science Foundation of China[81102392] ; National Natural Science Foundation of China[81620108028] ; National Natural Science Foundation of China[81473143] ; National Natural Science Foundation of China[81430087] ; Natural Science Foundation of Zhejiang Province[LZ14H300001] ; Zhejiang Provincial Program for the Cultivation of High -Level Innovative Health Talents[00000000] ; National Science and Technology Major Project[2013ZX09402103]
WOS研究方向Science & Technology - Other Topics ; Materials Science
语种英语
WOS记录号WOS:000393355900014
出版者AMER CHEMICAL SOC
源URL[http://119.78.100.183/handle/2S10ELR8/275654]  
专题分子影像中心（筹）
药物制剂研究中心
通讯作者Gao, Jian-Qing
作者单位1.Zhejiang Univ, Coll Chem & Biol Engn, Minist Educ, Key Lab Biomass Chem Engn, Hangzhou 310027, Zhejiang, Peoples R China
2.Zhejiang Univ, Coll Chem & Biol Engn, Minist Educ, Ctr Bionanoengn, Hangzhou 310027, Zhejiang, Peoples R China;
3.Kyoto Univ, Inst Frontier Med Sci, Dept Biomat, Field Tissue Engn, Kyoto 6068507, Japan;
4.Chinese Acad Sci, Shanghai Inst Mat Med, Ctr Drug Delivery Syst, Shanghai 201210, Peoples R China;
5.Zhejiang Univ, Dr Li Dak Sum & Yip Yio Chin Ctr Stem Cell & Rege, Hangzhou 310058, Zhejiang, Peoples R China;
6.Zhejiang Univ, Zhejiang Prov Key Lab Neurobiol, Key Lab Med Neurobiol, Dept Pharmacol,Minist Hlth China,Coll Pharmaceut, Hangzhou 310058, Zhejiang, Peoples R China;
7.Zhejiang Univ, Sch Med, Ctr Stem Cell & Tissue Engn, Hangzhou 310058, Zhejiang, Peoples R China;
8.Zhejiang Univ, Coll Pharmaceut Sci, Hangzhou 310058, Zhejiang, Peoples R China;
9.Zhejiang Univ, Coll Pharmaceut Sci, Inst Pharmaceut, Hangzhou 310058, Zhejiang, Peoples R China;
推荐引用方式
GB/T 7714		 Li, Li-Ming,Han, Min,Jiang, Xin-Chi,et al. Peptide-Tethered Hydrogel Scaffold Promotes Recovery from Spinal Cord Transection via Synergism with Mesenchymal Stem Cells[J]. ACS APPLIED MATERIALS & INTERFACES,2017,9(4):3330-3342.
APA Li, Li-Ming.,Han, Min.,Jiang, Xin-Chi.,Yin, Xian-Zhen.,Chen, Fu.,...&Gao, Jian-Qing.(2017).Peptide-Tethered Hydrogel Scaffold Promotes Recovery from Spinal Cord Transection via Synergism with Mesenchymal Stem Cells.ACS APPLIED MATERIALS & INTERFACES,9(4),3330-3342.
MLA Li, Li-Ming,et al."Peptide-Tethered Hydrogel Scaffold Promotes Recovery from Spinal Cord Transection via Synergism with Mesenchymal Stem Cells".ACS APPLIED MATERIALS & INTERFACES 9.4(2017):3330-3342.

入库方式： OAI收割

来源：上海药物研究所

浏览0
下载0
收藏0
其他版本

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。