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High-throughput measurement of drug-cyclodextrin kinetic rate constants by a small molecule microarray using surface plasmon resonance imaging

文献类型：期刊论文

作者Singh, Vikramjeet1,3; Li, Zhuo1,4; Zhou, Xiaotong1; Xu, Xiaonan1; Xu, Jianghui1; Nand, Amita3; Wen, Huajie2; Li, Haiyan1; Zhu, Jingsong2,3; Zhang, Jiwen1
刊名RSC ADVANCES
出版日期2016
卷号6期号:4页码:3213-3218
ISSN号2046-2069
DOI10.1039/c5ra21298g
文献子类Article
英文摘要Applications of small molecule microarrays (SMMs) has been extensively studied but have been limited to the screening of small molecule inhibitors. Here for the first time, we conjugated SMMs with label free surface plasmon resonance imaging (SPRi) for measurement of kinetic parameters for drug-cyclodextrins interactions in high-throughput manner. A collection of insoluble drugs was immobilized onto biosensor surface using photo-cross-linked technique to form SMMs. A highly sensitive and recently reported surface chemistry based on surface initiated polymerization chemistry was used for SMMs fabrication. In total, 38 insoluble drugs were evaluated for their interaction profile and kinetic rate constants against 5 different types of cyclodextrins (CDs) including, alpha-CD, beta-CD, gamma-CD, 2-hydroxylpropyl beta-CD (HP-beta-CD) and sulphobutyl-ether-beta-CD (SBE-beta-CD). For the supramolecular drug-CD interaction kinetics, the response magnitude and detailed kinetic parameters were calculated and presented in the article. The presented method described a label free and high-throughput technique for real time measurement of kinetic constants for drug-CDs interactions which will assist the selection and use of different CDs in number of different applications.
WOS关键词FLUORESCENCE CORRELATION SPECTROSCOPY ; BIOLOGICAL-MEMBRANES ; BIACORE(R) SYSTEM ; COMPLEXES ; DYNAMICS ; TIME
资助项目Natural Science Foundation of China[81373358] ; Natural Science Foundation of China[81430087]
WOS研究方向Chemistry
语种英语
WOS记录号WOS:000368193500079
出版者ROYAL SOC CHEMISTRY
源URL[http://119.78.100.183/handle/2S10ELR8/276265]  
专题药物制剂研究中心
通讯作者Zhang, Jiwen
作者单位1.Chinese Acad Sci, Shanghai Inst Mat Med, Ctr Drug Delivery Syst, Shanghai 201203, Peoples R China;
2.Guangzhou Gaotong Biol Technol Co Ltd, Guangzhou 510663, Guangdong, Peoples R China
3.Natl Ctr Nanosci & Technol, Beijing 100190, Peoples R China;
4.Shenyang Pharmaceut Univ, Sch Pharm, Shenyang 110016, Peoples R China;
推荐引用方式
GB/T 7714		 Singh, Vikramjeet,Li, Zhuo,Zhou, Xiaotong,et al. High-throughput measurement of drug-cyclodextrin kinetic rate constants by a small molecule microarray using surface plasmon resonance imaging[J]. RSC ADVANCES,2016,6(4):3213-3218.
APA Singh, Vikramjeet.,Li, Zhuo.,Zhou, Xiaotong.,Xu, Xiaonan.,Xu, Jianghui.,...&Zhang, Jiwen.(2016).High-throughput measurement of drug-cyclodextrin kinetic rate constants by a small molecule microarray using surface plasmon resonance imaging.RSC ADVANCES,6(4),3213-3218.
MLA Singh, Vikramjeet,et al."High-throughput measurement of drug-cyclodextrin kinetic rate constants by a small molecule microarray using surface plasmon resonance imaging".RSC ADVANCES 6.4(2016):3213-3218.

入库方式： OAI收割

来源：上海药物研究所

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