Bioactivity assay of porcine relaxin based on cAMP accumulation in THP-1 cells quantified by LC-MS/MS
文献类型：期刊论文
| 作者 | Ren, Xiaohong3,4; Wang, Caifen2,3; Wu, Li3,4; Li, Haiyan3 ; Chi, Liandi3; Sun, Lixin2; Wang, Bin1; Cui, Hu1; Angiolini, Luca5; Bianchi, Alessandro5
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| 刊名 | JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
 |
| 出版日期 | 2015-07-10 |
| 卷号 | 111页码:320-323 |
| 关键词 | Porcine relaxin Bioactivity cAMP LC-MS/MS ELISA |
| ISSN号 | 0731-7085 |
| DOI | 10.1016/j.jpba.2015.04.016 |
| 文献子类 | Article |
| 英文摘要 | This study describes a reliable bioactivity assay of porcine relaxin (pRIX) based on cyclic adenosine 3',5'-monophosphate (cAMP) accumulation in the human monocyte cell-line quantified by liquid chromatography-tandem mass spectrometry (LC-MS/MS). As a result, the LC-MS/MS was based on a positive selected reaction monitoring of cAMP with a stable internal standard, 8-Br-cAMP and a protein precipitation procedure by HClO4. The standard curve of cAMP was linear from 5.0 ng mL(-1) to 992.0 ngmL(-1), with lower limits of detection and quantification of 0.5 ng mL-1 and 5.0 ng mL(-1), respectively. The satisfactory validation data including stability assay were obtained. When measured by the LC-MS/MS, the pRIX sample showed a time- and dose-dependent stimulation of cAMP with the concentration for 50% of the maximal effect (EC50) of 40.6 ng mL(-1). The developed method indicated higher precision and selectivity than the commercial enzyme linked immunosorbent assay kits, which showed EC50 of 66.6 ng mL(-1). (C) 2015 Elsevier B.V. All rights reserved. |
| 资助项目 | National Natural Science Foundation of China[81373358] |
| WOS研究方向 | Chemistry ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:000355359100042 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/276471]  |
| 专题 | 药物制剂研究中心 |
| 通讯作者 | Zhang, Jiwen |
| 作者单位 | 1.Rizhao Lanshan Biochem Prod Co Ltd, Rizhao 276800, Peoples R China; 2.Shenyang Pharmaceut Univ, Sch Pharm, Shenyang 110016, Peoples R China; 3.Chinese Acad Sci, Shanghai Inst Mat Med, Ctr Drug Delivery Syst, Shanghai 201203, Peoples R China; 4.Jilin Univ, Coll Life Sci, Edmond H Fischer Signal Transduct Lab, Changchun 130012, Peoples R China; 5.IBSA Inst Biochim SA, CH-6574 Lugano, Switzerland |
| 推荐引用方式 GB/T 7714 | Ren, Xiaohong,Wang, Caifen,Wu, Li,et al. Bioactivity assay of porcine relaxin based on cAMP accumulation in THP-1 cells quantified by LC-MS/MS[J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,2015,111:320-323. |
| APA | Ren, Xiaohong.,Wang, Caifen.,Wu, Li.,Li, Haiyan.,Chi, Liandi.,...&Zhang, Jiwen.(2015).Bioactivity assay of porcine relaxin based on cAMP accumulation in THP-1 cells quantified by LC-MS/MS.JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,111,320-323. |
| MLA | Ren, Xiaohong,et al."Bioactivity assay of porcine relaxin based on cAMP accumulation in THP-1 cells quantified by LC-MS/MS".JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS 111(2015):320-323. |
入库方式： OAI收割
来源：上海药物研究所
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