Synchrotron radiation-based Fourier-transform infrared spectromicroscopy for characterization of the protein/peptide distribution in single microspheres
文献类型：期刊论文
| 作者 | Wang, Manli1,2; Lu, Xiaolong2,3; Yin, Xianzhen2; Tong, Yajun4; Peng, Weiwei4; Wu, Li2,5; Li, Haiyan2 ; Yang, Yan5; Gu, Jingkai5; Xiao, Tiqiao4
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| 刊名 | ACTA PHARMACEUTICA SINICA B
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| 出版日期 | 2015-05 |
| 卷号 | 5期号:3页码:270-276 |
| 关键词 | Fourier-transform infrared spectromicroscopy Micerosphere Exenatide PLGA |
| ISSN号 | 2211-3835 |
| DOI | 10.1016/j.apsb.2015.03.008 |
| 文献子类 | Article |
| 英文摘要 | The present study establishes a visualization method for the measurement of the distribution and localization of protein/peptide constituents within a single poly-lactide-co-glycolide (PLGA) microsphere using synchrotron radiation based Fourier-transform infrared speciiomlcroscopy (SR-FTIR). The representative infrared wavenumbers specific for protein/peptide (Exenatide) and excipient (PLGA) were identified and chemical maps at the single microsphere level were generated by measuring and plotting the intensity of these specific bands. For quantitative analysis of the distribution within microspheres, Matlab soft are was used to transform the map file into a 3D matrix and the matiix values specific for the drug and excipient were extracted. Comparison of the normalized SR- FM maps of PLGA and Exenatide indicated that PLGA was unit-on-lily distributed, while Exenatide was relatively non-uniformly distributed in the microspheres. In conclusion, SR-FTIR is a rapid, nondestructive and sensitive detection technology to provide the distribution of chemical constituents and functional groups in microparticles and microspheres. (C) 2015 Chinese Pharmaceutical Association and Institute of Materia 'Medico, Chinese Academy of 'Medical Sciences. Production and hosting by Elsevier B.V. |
| WOS关键词 | FTIR SPECTROMICROSCOPY ; FLUORESCENCE MICROSCOPY ; RELEASE BEHAVIOR ; DRUG-DELIVERY ; MICROSPECTROSCOPY ; NANOPARTICLES ; PROTEIN ; MECHANISM ; CELLS |
| 资助项目 | National Natural Science Foundation of China[81273453] ; National Natural Science Foundation of China[81430087] |
| WOS研究方向 | Pharmacology & Pharmacy |
| 语种 | 英语 |
| CSCD记录号 | CSCD:5448877 |
| WOS记录号 | WOS:000360008900014 |
| 出版者 | INST MATERIA MEDICA, CHINESE ACAD MEDICAL SCIENCES |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/276541]  |
| 专题 | 药物制剂研究中心 分子影像中心（筹） |
| 通讯作者 | Chen, Min |
| 作者单位 | 1.Anhui Univ Chinese Med, Sch Pharmaceut Sci, Hefei 230038, Peoples R China; 2.Chinese Acad Sci, Ctr Pharmaceut Preparat, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China; 3.Shanghai Inst Technol, Sch Mech Engn, Shanghai 201418, Peoples R China; 4.Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai Synchrotron Radiat Facil, Shanghai 201204, Peoples R China; 5.Jilin Univ, Coll Life Sci, Changchun 130012, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Manli,Lu, Xiaolong,Yin, Xianzhen,et al. Synchrotron radiation-based Fourier-transform infrared spectromicroscopy for characterization of the protein/peptide distribution in single microspheres[J]. ACTA PHARMACEUTICA SINICA B,2015,5(3):270-276. |
| APA | Wang, Manli.,Lu, Xiaolong.,Yin, Xianzhen.,Tong, Yajun.,Peng, Weiwei.,...&Zhang, Jiwen.(2015).Synchrotron radiation-based Fourier-transform infrared spectromicroscopy for characterization of the protein/peptide distribution in single microspheres.ACTA PHARMACEUTICA SINICA B,5(3),270-276. |
| MLA | Wang, Manli,et al."Synchrotron radiation-based Fourier-transform infrared spectromicroscopy for characterization of the protein/peptide distribution in single microspheres".ACTA PHARMACEUTICA SINICA B 5.3(2015):270-276. |
入库方式： OAI收割
来源：上海药物研究所
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