Microneedle-assisted dendritic cell-targeted nanoparticles for transcutaneous DNA immunization
文献类型：期刊论文
| 作者 | Hu, Ying1,2; Xu, Beihua2; Xu, Jiaojiao2,3; Shou, Dan4; Liu, Ergang5; Gao, Jianqing1; Liang, Wenquan1; Huang, Yongzhuo5
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| 刊名 | POLYMER CHEMISTRY
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| 出版日期 | 2015-01-21 |
| 卷号 | 6期号:3页码:373-379 |
| ISSN号 | 1759-9954 |
| DOI | 10.1039/c4py01394h |
| 文献子类 | Article |
| 英文摘要 | Transcutaneous immunization (TCI) is an attractive vaccination strategy for targeting the epidermal dendritic cells (DCs). TCI has been widely explored for protection against infectious diseases. Recently, its promising application in cancer immunotherapy has also attracted great attention. However, effective percutaneous delivery of the vaccines must overcome the formidable stratum corneum, as well as specifically target DCs; both are big challenges. We developed the mannosylated grafted cell-penetrating peptide-low molecular weight PEI copolymer (CPP-PEI1800-Man) with the combination of microneedles for DC-targeting percutaneous delivery of DNA vaccine for malignant melanoma (MM) therapy. Mannose receptors are overexpressed on the surface of DCs. The microneedle-assisted in vivo skin penetration of the CPP-PEI1800-Man/DNA nanoparticles was investigated, and the DC-targeting efficiency was measured. The induction of protective and therapeutic anti-tumor immunity by the CPP-PEI1800-Man/DNA nanoparticles was significantly enhanced compared to the PEI25k/DNA vaccines. Transcutaneous vaccination with the microneedle-assisted CPP-PEI1800-Man/DNA efficiently promoted Trp2-specific cellular immune responses, resulting in effective protection against B16 melanoma challenge in BALB/c mice. Importantly, the CPP-PEI1800-Man/DNA nanoparticles strongly induced CD8(+) cytotoxic T lymphocyte activity and CD4(+) T cells that secreted the interferon-gamma. and interleukin-12 cytokines against melanoma cells. As a result, cancer growth was inhibited and the survival time of B16-xenografted BALB/c mice was prolonged. Therefore, the CPP-PEI1800-Man copolymer is promising for DC-specific vaccination, and microneedle-assisted CPP-PEI1800-Man/DNA delivery represents a potential immunotherapeutic strategy for MM. |
| WOS关键词 | TRANSDERMAL DRUG-DELIVERY ; ANTITUMOR IMMUNITY ; CANCER-IMMUNOTHERAPY ; GENE DELIVERY ; IN-VITRO ; VACCINE ; MELANOMA ; SKIN ; POLYETHYLENIMINE ; INDUCTION |
| 资助项目 | Natural Science Foundation of China (NSFC)[30973648] ; Natural Science Foundation of China (NSFC)[81422048] ; Natural Science Foundation of Zhejiang Province, China[LY12H30002] ; Natural Science Foundation of Zhejiang Province, China[Z14H300001] |
| WOS研究方向 | Polymer Science |
| 语种 | 英语 |
| WOS记录号 | WOS:000346422300006 |
| 出版者 | ROYAL SOC CHEMISTRY |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/276668]  |
| 专题 | 药物制剂研究中心 |
| 通讯作者 | Huang, Yongzhuo |
| 作者单位 | 1.Zhejiang Univ, Coll Pharmaceut Sci, Hangzhou 310003, Zhejiang, Peoples R China; 2.Zhejiang Pharmaceut Coll, Ningbo, Zhejiang, Peoples R China; 3.Wenzhou Med Univ, Dept Med, Wenzhou, Zhejiang, Peoples R China; 4.Zhejiang Acad Tradit Chinese Med, Dept Med, Hangzhou, Zhejiang, Peoples R China; 5.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Hu, Ying,Xu, Beihua,Xu, Jiaojiao,et al. Microneedle-assisted dendritic cell-targeted nanoparticles for transcutaneous DNA immunization[J]. POLYMER CHEMISTRY,2015,6(3):373-379. |
| APA | Hu, Ying.,Xu, Beihua.,Xu, Jiaojiao.,Shou, Dan.,Liu, Ergang.,...&Huang, Yongzhuo.(2015).Microneedle-assisted dendritic cell-targeted nanoparticles for transcutaneous DNA immunization.POLYMER CHEMISTRY,6(3),373-379. |
| MLA | Hu, Ying,et al."Microneedle-assisted dendritic cell-targeted nanoparticles for transcutaneous DNA immunization".POLYMER CHEMISTRY 6.3(2015):373-379. |
入库方式： OAI收割
来源：上海药物研究所
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