Spray-dried powders enhance vaginal siRNA delivery by potentially modulating the mucus molecular sieve structure
文献类型：期刊论文
| 作者 | Wu, Na1,2; Zhang, Xinxin2 ; Li, Feifei2; Zhang, Tao2; Gan, Yong2; Li, Juan1
|
| 刊名 | INTERNATIONAL JOURNAL OF NANOMEDICINE
 |
| 出版日期 | 2015 |
| 卷号 | 10页码:5383-5396 |
| 关键词 | siRNA delivery vaginal administration spray-dried powders mucus penetration molecular sieve effect |
| ISSN号 | 1178-2013 |
| DOI | 10.2147/IJN.S87978 |
| 文献子类 | Article |
| 英文摘要 | Vaginal small interfering RNA (siRNA) delivery provides a promising strategy for the prevention and treatment of vaginal diseases. However, the densely cross-linked mucus layer on the vaginal wall severely restricts nanoparticle-mediated siRNA delivery to the vaginal epithelium. In order to overcome this barrier and enhance vaginal mucus penetration, we prepared spray-dried powders containing siRNA-loaded nanoparticles. Powders with Pluronic F127 (F127), hydroxypropyl methyl cellulose (HPMC), and mannitol as carriers were obtained using an ultrasound-assisted spray-drying technique. Highly dispersed dry powders with diameters of 5-15 mu m were produced. These powders showed effective siRNA protection and sustained release. The mucus-penetrating properties of the powders differed depending on their compositions. They exhibited different potential of opening mesh size of molecular sieve in simulated vaginal mucus system. A powder formulation with 0.6% F127 and 0.1% HPMC produced the maximum increase in the pore size of the model gel used to simulate vaginal mucus by rapidly extracting water from the gel and interacting with the gel; the resulting modulation of the molecular sieve effect achieved a 17.8-fold improvement of siRNA delivery in vaginal tract and effective siRNA delivery to the epithelium. This study suggests that powder formulations with optimized compositions have the potential to alter the steric barrier posed by mucus and hold promise for effective vaginal siRNA delivery. |
| WOS关键词 | VITRO DNA DELIVERY ; DRUG-DELIVERY ; POLYMERIC NANOPARTICLES ; GEL FORMULATIONS ; IN-VIVO ; SYSTEMS ; INFECTION ; LIPIDOIDS ; MATRIX ; FLOWS |
| 资助项目 | CAS equipment development project[YZ201244] ; Natural Science Foundation of China[81373356] ; Youth Innovation Promotion Association, CAS[00000000] |
| WOS研究方向 | Science & Technology - Other Topics ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:000360058900001 |
| 出版者 | DOVE MEDICAL PRESS LTD |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/276712]  |
| 专题 | 药物制剂研究中心 |
| 通讯作者 | Gan, Yong |
| 作者单位 | 1.China Pharmaceut Univ, Sch Pharm, Nanjing, Jiangsu, Peoples R China; 2.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wu, Na,Zhang, Xinxin,Li, Feifei,et al. Spray-dried powders enhance vaginal siRNA delivery by potentially modulating the mucus molecular sieve structure[J]. INTERNATIONAL JOURNAL OF NANOMEDICINE,2015,10:5383-5396. |
| APA | Wu, Na,Zhang, Xinxin,Li, Feifei,Zhang, Tao,Gan, Yong,&Li, Juan.(2015).Spray-dried powders enhance vaginal siRNA delivery by potentially modulating the mucus molecular sieve structure.INTERNATIONAL JOURNAL OF NANOMEDICINE,10,5383-5396. |
| MLA | Wu, Na,et al."Spray-dried powders enhance vaginal siRNA delivery by potentially modulating the mucus molecular sieve structure".INTERNATIONAL JOURNAL OF NANOMEDICINE 10(2015):5383-5396. |
入库方式： OAI收割
来源：上海药物研究所
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