In vitro and in vivo evaluation of chitosan graft glyceryl monooleate as peroral delivery carrier of enoxaparin
文献类型：期刊论文
| 作者 | Wang, Linlin2; Li, Liang2 ; Sun, Yujiao2; Ding, Jiaojiao2; Li, Jinfeng2; Duan, Xiaopin2; Li, Yaping1; Junyaprasert, Varaporn B.3; Mao, Shirui2
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| 刊名 | INTERNATIONAL JOURNAL OF PHARMACEUTICS
 |
| 出版日期 | 2014-08-25 |
| 卷号 | 471期号:1-2页码:391-399 |
| 关键词 | Chitosan Glyceryl monooleate Oral Enoxaparin Polyelectrolyte nanocomplexes |
| ISSN号 | 0378-5173 |
| DOI | 10.1016/j.ijpharm.2014.05.050 |
| 文献子类 | Article |
| 英文摘要 | In this paper a novel copolymer, chitosan graft glyceryl monooleate (CS-GO) was synthesized and its potential as the nanocarrier for enhancing the peroral delivery of enoxaparin was studied systemically. The successful synthesis was characterized by H-1 NMR. Enoxaparin nanocomplexes were prepared by self-assembly. Mucoadhesive properties of the nanocomplexes were evaluated using mucin particle method. Uptake and transport of the nanocomplexes were investigated in Caco-2 cells. In vivo absorption was studied in rats. The therapeutic effects of the nanocomplexes were evaluated using pulmonary thromboembolism model in mice. This study demonstrated that compared to chitosan based system, hydrophobic modification of CS with GO enhanced the oral absorption of enoxaparin significantly, which is in good agreement with the enhanced mucoadhesion, cellular internalization and transport in cell culture. Cellular uptake of CS-GO based enoxaparin nanocomplexes was incubation time, enoxaparin concentration and incubation temperature dependent. The uptake mechanism was assumed to be adsorptive endocytosis via clathrin- and caveolae-mediated process. Its therapeutic efficacy was further demonstrated by pharmacodynamic study with pulmonary thromboembolism inhibition percentage 47.1%. In conclusion, CS-GO copolymer is a promising nanocarrier for enhancing the oral absorption of enoxaparin. (C) 2014 Elsevier B.V. All rights reserved. |
| WOS关键词 | ORAL DELIVERY ; GENE DELIVERY ; PHYSICOCHEMICAL PROPERTIES ; DRUG-DELIVERY ; DNA DELIVERY ; NANOPARTICLES ; INSULIN ; ACID ; NANOCOMPLEXES ; TRANSFECTION |
| 资助项目 | Natural Science Foundation of China[81273446] ; Sino-Thai Joint Research and Development Project[9-503J] ; Ministry of Science and Technology of the People's Republic of China[00000000] |
| WOS研究方向 | Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:000338423600046 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/276937]  |
| 专题 | 药物制剂研究中心 |
| 通讯作者 | Mao, Shirui |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, Ctr Pharmaceut, Shanghai 201203, Peoples R China; 2.Shenyang Pharmaceut Univ, Sch Pharm, Shenyang 110016, Peoples R China; 3.Mahidol Univ, Dept Pharm, Fac Pharm, Bangkok 10400, Thailand |
| 推荐引用方式 GB/T 7714 | Wang, Linlin,Li, Liang,Sun, Yujiao,et al. In vitro and in vivo evaluation of chitosan graft glyceryl monooleate as peroral delivery carrier of enoxaparin[J]. INTERNATIONAL JOURNAL OF PHARMACEUTICS,2014,471(1-2):391-399. |
| APA | Wang, Linlin.,Li, Liang.,Sun, Yujiao.,Ding, Jiaojiao.,Li, Jinfeng.,...&Mao, Shirui.(2014).In vitro and in vivo evaluation of chitosan graft glyceryl monooleate as peroral delivery carrier of enoxaparin.INTERNATIONAL JOURNAL OF PHARMACEUTICS,471(1-2),391-399. |
| MLA | Wang, Linlin,et al."In vitro and in vivo evaluation of chitosan graft glyceryl monooleate as peroral delivery carrier of enoxaparin".INTERNATIONAL JOURNAL OF PHARMACEUTICS 471.1-2(2014):391-399. |
入库方式： OAI收割
来源：上海药物研究所
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