Uptake, transport and peroral absorption of fatty glyceride grafted chitosan copolymer-enoxaparin nanocomplexes: Influence of glyceride chain length
文献类型：期刊论文
| 作者 | Wang, Linlin1; Sun, Yujiao1; Shi, Chenjun1; Li, Liang1 ; Guan, Jian1; Zhang, Xin1; Ni, Rui1; Duan, Xiaopin1; Li, Yaping2; Mao, Shirui1
|
| 刊名 | ACTA BIOMATERIALIA
 |
| 出版日期 | 2014-08 |
| 卷号 | 10期号:8页码:3675-3685 |
| 关键词 | Chitosan Fatty glyceride Oral Enoxaparin Polyelectrolyte nanocomplexes |
| ISSN号 | 1742-7061 |
| DOI | 10.1016/j.actbio.2014.05.003 |
| 文献子类 | Article |
| 英文摘要 | The objective of this paper is to elucidate the influence of fatty glyceride chain length in chitosan copolymers on the peroral absorption of enoxaparin. First of all, a series of chitosan copolymers with glyceryl monocaprylate (GM8), glyceryl monolaurate (GM12) and glyceryl monostearate (GM18) as the hydrophobic part were synthesized. The structure of the copolymers was characterized using proton nuclear magnetic resonance. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay demonstrated that all the copolymers were non-toxic. Enoxaparin nanocomplexes were prepared by self-assembly. Mucoadhesion of the nanocomplexes was characterized using the mucin particle method. Nanocomplex uptake and transport were quantified in Caco-2 cells and cellular localization was visualized by confocal laser scanning microscopy. Enoxaparin uptake was enhanced by nanocomplex formation, and was dependent on incubation time, concentration, temperature and glyceride chain length. The GM8 grafted chitosan-enoxaparin nanocomplex exhibited the strongest bioadhesion and the best uptake and transport in both cell culture and in vivo absorption in rats. The uptake mechanism was assumed to be adsorptive endocytosis via clathrin- and caveolae-mediated processes. In conclusion, oral absorption of enoxaparin can be further enhanced by using GM8 grafted chitosan copolymer as the carrier to form nanocomplexes. (C) 2014 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved. |
| WOS关键词 | DRUG-DELIVERY ; ORAL DELIVERY ; ENDOCYTIC PATHWAYS ; EPITHELIAL-CELLS ; CELLULAR UPTAKE ; IN-VITRO ; NANOPARTICLES ; INSULIN ; HEPARIN ; TRANSFECTION |
| 资助项目 | Natural Science Foundation of China[81273446] ; Sino-Thai joint research and development project, the Ministry of Science and Technology of the PR China[19-503J] |
| WOS研究方向 | Engineering ; Materials Science |
| 语种 | 英语 |
| WOS记录号 | WOS:000339459500030 |
| 出版者 | ELSEVIER SCI LTD |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/276973]  |
| 专题 | 药物制剂研究中心 |
| 通讯作者 | Mao, Shirui |
| 作者单位 | 1.Shenyang Pharmaceut Univ, Sch Pharm, Shenyang 110016, Peoples R China; 2.Chinese Acad Sci, Shanghai Inst Mat Med, Ctr Pharmaceut, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Linlin,Sun, Yujiao,Shi, Chenjun,et al. Uptake, transport and peroral absorption of fatty glyceride grafted chitosan copolymer-enoxaparin nanocomplexes: Influence of glyceride chain length[J]. ACTA BIOMATERIALIA,2014,10(8):3675-3685. |
| APA | Wang, Linlin.,Sun, Yujiao.,Shi, Chenjun.,Li, Liang.,Guan, Jian.,...&Mao, Shirui.(2014).Uptake, transport and peroral absorption of fatty glyceride grafted chitosan copolymer-enoxaparin nanocomplexes: Influence of glyceride chain length.ACTA BIOMATERIALIA,10(8),3675-3685. |
| MLA | Wang, Linlin,et al."Uptake, transport and peroral absorption of fatty glyceride grafted chitosan copolymer-enoxaparin nanocomplexes: Influence of glyceride chain length".ACTA BIOMATERIALIA 10.8(2014):3675-3685. |
入库方式： OAI收割
来源：上海药物研究所
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