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Effective delivery of p65 shRNA by optimized Tween 85-polyethyleneimine conjugate for inhibition of tumor growth and lymphatic metastasis

文献类型：期刊论文

作者Xiao, Jisheng2; Duan, Xiaopin1,2; Meng, Qingshuo2; Yin, Qi2; Zhang, Zhiwen2; Yu, Haijun2; Chen, Lingli2; Gu, Wangwen2; Li, Yaping2
刊名ACTA BIOMATERIALIA
出版日期2014-06
卷号10期号:6页码:2674-2683
关键词Tween 85-PEI conjugates p65 shRNA Lymphatic metastasis Breast cancer Nanoparticles
ISSN号1742-7061
DOI10.1016/j.actbio.2014.02.009
文献子类Article
英文摘要To maximize the interference efficacy of pGPU6/Neo-p65 shRNA-expressing pDNA (p65 shRNA) and subsequently more effectively inhibit tumor growth and lymphatic metastasis through blocking the nuclear factor-kappa B (NF-kappa B) signaling pathway, seven Tween 85-polyethyleneimine (PEI) conjugates (TnPs, n = 2, 3, 4, 5, 6, 7 and 8), which differed in the length of the polymethylene [-(CH2)(n)-] spacer between Tween 85 and PEI, were synthesized and investigated. The results showed that the transfection efficiency and cytotoxicity both increased with the spacer chain length. Then, TnPs with a [-(CH2)(6)-] spacer (T6P) were chosen to deliver p65 shRNA to a tumor and subsequently inhibit tumor growth and lymphatic metastasis. The T6P/p65 shRNA complex nanoparticles (T(6)Ns) could significantly down-regulate p65 expression in breast cancer cells, and consequently inhibit cell invasion and disrupt the tube formation. Most importantly, T(6)Ns accumulated greatly in tumor tissue, and as a result, significantly inhibited the growth and lymphatic metastasis of breast cancer xenograft. All these results indicated that the transfection efficacies of cationic amphiphiles could be significantly modulated by minor structural variations, and that T6P was promising for the effective delivery of p65 shRNA to knock down the expression of the key metastasis-driving genes and inhibit tumor growth and metastasis. (C) 2014 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
WOS关键词NF-KAPPA-B ; GENE-TRANSFER EFFICACIES ; BREAST-CANCER ; TRANSFECTION PROPERTIES ; CATIONIC AMPHIPHILES ; SERUM COMPATIBILITY ; INVASION ; CELLS ; LIPIDS ; ANGIOGENESIS
资助项目National Basic Research Program of China[2010CB934000] ; National Basic Research Program of China[2013CB932503] ; National Natural Science Foundation of China[81230029] ; National Natural Science Foundation of China[81270047] ; Shanghai Program[12nm 0501300]
WOS研究方向Engineering ; Materials Science
语种英语
WOS记录号WOS:000336345900031
出版者ELSEVIER SCI LTD
源URL[http://119.78.100.183/handle/2S10ELR8/277054]  
专题药物制剂研究中心
通讯作者Li, Yaping
作者单位1.Shenyang Pharmaceut Univ, Sch Pharm, Shenyang 110016, Peoples R China
2.Chinese Acad Sci, Shanghai Inst Mat Med, Ctr Pharmaceut, Shanghai 201203, Peoples R China;
推荐引用方式
GB/T 7714		 Xiao, Jisheng,Duan, Xiaopin,Meng, Qingshuo,et al. Effective delivery of p65 shRNA by optimized Tween 85-polyethyleneimine conjugate for inhibition of tumor growth and lymphatic metastasis[J]. ACTA BIOMATERIALIA,2014,10(6):2674-2683.
APA Xiao, Jisheng.,Duan, Xiaopin.,Meng, Qingshuo.,Yin, Qi.,Zhang, Zhiwen.,...&Li, Yaping.(2014).Effective delivery of p65 shRNA by optimized Tween 85-polyethyleneimine conjugate for inhibition of tumor growth and lymphatic metastasis.ACTA BIOMATERIALIA,10(6),2674-2683.
MLA Xiao, Jisheng,et al."Effective delivery of p65 shRNA by optimized Tween 85-polyethyleneimine conjugate for inhibition of tumor growth and lymphatic metastasis".ACTA BIOMATERIALIA 10.6(2014):2674-2683.

入库方式： OAI收割

来源：上海药物研究所

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