Reversal of Lung Cancer Multidrug Resistance by pH-Responsive Micelleplexes Mediating Co-Delivery of siRNA and Paclitaxel
文献类型：期刊论文
| 作者 | Yu, Haijun1 ; Xu, Zhiai2; Chen, Xianzhi1; Xu, Leilei3; Yin, Qi1; Zhang, Zhiwen1; Li, Yaping1
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| 刊名 | MACROMOLECULAR BIOSCIENCE
 |
| 出版日期 | 2014-01 |
| 卷号 | 14期号:1页码:100-109 |
| 关键词 | lung cancer micelleplexes multidrug resistance Paclitaxel pH-responsive siRNA |
| ISSN号 | 1616-5187 |
| DOI | 10.1002/mabi.201300282 |
| 文献子类 | Article |
| 英文摘要 | The recent advances in RNA interference (RNAi) technology provided novel and promising solutions for human cancer treatment. In this study, the application of dual pH-responsive cationic micellar nanoparticles for small interfering RNA (siRNA) and paclitaxel (PTX) co-delivery to overcome cancer multidrug resistance (MDR) is reported. The in vitro siRNA transfection shows that siRNA-luciferase (Luc) loaded micelleplexes efficiently silences Luc expression in various carcinoma cell lines. The Luc knockdown ability of the micelleplexes can be enhanced by choloquine (CQ) co-incubation. However, is abolished by bafilomycin-A1 (Baf-A1) treatment. The micelleplexes are further exploited for co-delivery of siRNA-Bcl-2 and PTX to Bcl-2 overexpressing A549 lung cancer cells (A549-Bcl-2). The experimental results show that the micelleplexes could sensitize A549-Bcl-2 cells to PTX via down-regulation of anti-apoptosis gene of Bcl-2, suggesting that PDMA-b-PDPA micelleplexes are promising nanovectors for siRNA and anti-cancer drug co-delivery to overcome cancer MDR. |
| WOS关键词 | TRIBLOCK COPOLYMERS ; GENE DELIVERY ; APOPTOSIS ; BCL-2 ; RNA ; DNA ; TRANSFECTION ; POLYPLEXES ; EXPRESSION ; DIBLOCK |
| 资助项目 | National Basic Research Program of China[2013CB932704] ; National Basic Research Program of China[2010CB934000] ; National Basic Research Program of China[2012CB932502] ; National Natural Science Foundation of China[81230029] ; National Natural Science Foundation of China[81270047] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Materials Science ; Polymer Science |
| 语种 | 英语 |
| WOS记录号 | WOS:000330806100008 |
| 出版者 | WILEY-V C H VERLAG GMBH |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/277316]  |
| 专题 | 药物制剂研究中心 |
| 通讯作者 | Li, Yaping |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, Ctr Pharmaceut, Shanghai 201203, Peoples R China; 2.E China Normal Univ, Dept Chem, Shanghai 200241, Peoples R China; 3.Jiangsu Univ, Sch Pharm, Zhenjiang 202013, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yu, Haijun,Xu, Zhiai,Chen, Xianzhi,et al. Reversal of Lung Cancer Multidrug Resistance by pH-Responsive Micelleplexes Mediating Co-Delivery of siRNA and Paclitaxel[J]. MACROMOLECULAR BIOSCIENCE,2014,14(1):100-109. |
| APA | Yu, Haijun.,Xu, Zhiai.,Chen, Xianzhi.,Xu, Leilei.,Yin, Qi.,...&Li, Yaping.(2014).Reversal of Lung Cancer Multidrug Resistance by pH-Responsive Micelleplexes Mediating Co-Delivery of siRNA and Paclitaxel.MACROMOLECULAR BIOSCIENCE,14(1),100-109. |
| MLA | Yu, Haijun,et al."Reversal of Lung Cancer Multidrug Resistance by pH-Responsive Micelleplexes Mediating Co-Delivery of siRNA and Paclitaxel".MACROMOLECULAR BIOSCIENCE 14.1(2014):100-109. |
入库方式： OAI收割
来源：上海药物研究所
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