Intestinal mucosa permeability following oral insulin delivery using core shell corona nanolipoparticles
文献类型：期刊论文
| 作者 | Li, Xiuying1,4; Guo, Shiyan1; Zhu, Chunliu1 ; Zhu, Quanlei1; Gan, Yong1; Rantanen, Jukka4; Rahbek, Ulrik Lytt3; Hovgaard, Lars2; Yang, Mingshi4
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| 刊名 | BIOMATERIALS
 |
| 出版日期 | 2013-12 |
| 卷号 | 34期号:37页码:9678-9687 |
| 关键词 | Oral protein delivery Core shell corona Insulin Mucus penetrating particles Cellular uptake |
| ISSN号 | 0142-9612 |
| DOI | 10.1016/j.biomaterials.2013.08.048 |
| 文献子类 | Article |
| 英文摘要 | Chitosan nanoparticles (NC) have excellent capacity for protein entrapment, favorable epithelial permeability, and are regarded as promising nanocarriers for oral protein delivery. Herein, we designed and evaluated a class of core shell corona nanolipoparticles (CSC) to further improve the absorption through enhanced intestinal mucus penetration. CSC contains chitosan nanoparticles as a core component and pluronic F127-lipid vesicles as a shell with hydrophilic chain and polyethylene oxide PEO as a corona. These particles were developed by hydration of a dry pluronic F127-lipid film with NC suspensions followed by extrusion. Insulin nested inside CSC was well protected from enzymatic degradation. Compared with NC, CSC exhibited significantly higher efficiency of mucosal penetration and, consequently, higher cellular internalization of insulin in mucus secreting E12 cells. The cellular level of insulin after CSC treatment was 36-fold higher compared to treatment with free insulin, and 10-fold higher compared to NC. CSC significantly facilitated the permeation of insulin across the ileum epithelia, as demonstrated in an ex vivo study and an in vivo absorption study. CSC pharmacological studies in diabetic rats showed that the hypoglycemic effects of orally administrated CSC were 2.5-fold higher compared to NC. In conclusion, CSC is a promising oral protein delivery system to enhance the stability, intestinal mucosal permeability, and oral absorption of insulin. (C) 2013 Elsevier Ltd. All rights reserved. |
| WOS关键词 | BIODEGRADABLE NANOPARTICLES ; EPITHELIAL-CELLS ; DRUG-DELIVERY ; IN-VITRO ; SYSTEMS ; MUCUS ; TRANSPORT ; MEMBRANE ; PROTEINS ; MONOLAYERS |
| 资助项目 | Novo Nordisk-Chinese Academy of Science (CAS) Research Foundation[NNCAS-2009-10] ; National Science and Technology Major Project, "Key New Drug Creation and Manufacturing Program"[2012ZX09301001-001] ; Innovation Consortium NanoMorph[952320/2009] ; Danish Council for Technology and Innovation[00000000] |
| WOS研究方向 | Engineering ; Materials Science |
| 语种 | 英语 |
| WOS记录号 | WOS:000326901200044 |
| 出版者 | ELSEVIER SCI LTD |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/277362]  |
| 专题 | 药物制剂研究中心 |
| 通讯作者 | Gan, Yong |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China; 2.Novo Nordisk AS, Oral Formulat Dev, DK-2760 Malov, Denmark 3.Novo Nordisk AS, ADME Dept, DK-2760 Malov, Denmark; 4.Univ Copenhagen, Fac Hlth & Med Sci, Dept Pharm, DK-2100 Copenhagen, Denmark; |
| 推荐引用方式 GB/T 7714 | Li, Xiuying,Guo, Shiyan,Zhu, Chunliu,et al. Intestinal mucosa permeability following oral insulin delivery using core shell corona nanolipoparticles[J]. BIOMATERIALS,2013,34(37):9678-9687. |
| APA | Li, Xiuying.,Guo, Shiyan.,Zhu, Chunliu.,Zhu, Quanlei.,Gan, Yong.,...&Yang, Mingshi.(2013).Intestinal mucosa permeability following oral insulin delivery using core shell corona nanolipoparticles.BIOMATERIALS,34(37),9678-9687. |
| MLA | Li, Xiuying,et al."Intestinal mucosa permeability following oral insulin delivery using core shell corona nanolipoparticles".BIOMATERIALS 34.37(2013):9678-9687. |
入库方式： OAI收割
来源：上海药物研究所
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