Co-delivery of paclitaxel and survivin shRNA by pluronic P85-PEI/TPGS complex nanoparticles to overcome drug resistance in lung cancer
文献类型：期刊论文
| 作者 | Shen, Jianan; Yin, Qi ; Chen, Lingli; Zhang, Zhiwen; Li, Yaping
|
| 刊名 | BIOMATERIALS
 |
| 出版日期 | 2012-11 |
| 卷号 | 33期号:33页码:8613-8624 |
| 关键词 | Drug resistance Co-delivery Paclitaxel Survivin shRNA Pluronic P85 |
| ISSN号 | 0142-9612 |
| DOI | 10.1016/j.biomaterials.2012.08.007 |
| 文献子类 | Article |
| 英文摘要 | Drug resistance is a main obstacle for the successful chemotherapy of lung cancer. In this work, a new co-delivery system, P85-PEI/TPGS/PTX/shSur complex nanoparticles (PTPNs), to overcome paclitaxel (PTX) resistance in A549 human lung cancer was designed and developed. The experimental results showed that PTPNs could facilitate drug into cells and induce survivin shRNA (shSur) into nuclei on A549 and A549/T cells, achieve efficient gene delivery and induce availably RNA interference on A549/T cells. The IC50 of PTPNs against A549/T cells was 360-fold lower than that of free PTX. The enhanced efficacy of PTPNs against A549/T cells was associated with PTX-induced apoptosis and cell arrest in G2/M phase. Down-regulation of survivin protein by PTPNs could lower the apoptosis threshold of drug resistant cells and render chemotherapeutic agents more effective. Moreover, the inhibition of GST activity by P85 was found to increase PTX accumulation in A549/T cells. The in vivo antitumor efficacy showed that PTPNs were more effective than that of the Taxol. As a result, the co-delivery of PTX and shSur by PTPNs could be a very powerful approach to improve the therapeutic effect of PTX in resistant lung cancer. (C) 2012 Elsevier Ltd. All rights reserved. |
| WOS关键词 | GENE DELIVERY ; MULTIDRUG-RESISTANCE ; IN-VIVO ; MIXED MICELLES ; CELLS ; TRANSPORTERS ; TRANSFERASE ; INHIBITION ; APOPTOSIS ; EFFICACY |
| 资助项目 | National Basic Research Program of China[2010CB934000] ; National Basic Research Program of China[2012CB932500] ; National Natural Science Foundation of China[30925041] ; National Natural Science Foundation of China[81102388] ; Shanghai Elitist Program[11XD1406200] |
| WOS研究方向 | Engineering ; Materials Science |
| 语种 | 英语 |
| WOS记录号 | WOS:000310401000040 |
| 出版者 | ELSEVIER SCI LTD |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/277892]  |
| 专题 | 药物制剂研究中心 |
| 通讯作者 | Li, Yaping |
| 作者单位 | Chinese Acad Sci, Ctr Pharmaceut, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Shen, Jianan,Yin, Qi,Chen, Lingli,et al. Co-delivery of paclitaxel and survivin shRNA by pluronic P85-PEI/TPGS complex nanoparticles to overcome drug resistance in lung cancer[J]. BIOMATERIALS,2012,33(33):8613-8624. |
| APA | Shen, Jianan,Yin, Qi,Chen, Lingli,Zhang, Zhiwen,&Li, Yaping.(2012).Co-delivery of paclitaxel and survivin shRNA by pluronic P85-PEI/TPGS complex nanoparticles to overcome drug resistance in lung cancer.BIOMATERIALS,33(33),8613-8624. |
| MLA | Shen, Jianan,et al."Co-delivery of paclitaxel and survivin shRNA by pluronic P85-PEI/TPGS complex nanoparticles to overcome drug resistance in lung cancer".BIOMATERIALS 33.33(2012):8613-8624. |
入库方式： OAI收割
来源：上海药物研究所
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