Overcoming multidrug resistance by co-delivery of Mdr-1 and survivin-targeting RNA with reduction-responsible cationic poly(beta-amino esters)
文献类型：期刊论文
| 作者 | Yin, Qi ; Shen, Jianan; Chen, Lingli; Zhang, Zhiwen; Gu, Wangwen; Li, Yaping
|
| 刊名 | BIOMATERIALS
 |
| 出版日期 | 2012-09 |
| 卷号 | 33期号:27页码:6495-6506 |
| 关键词 | Poly(beta-amino esters) RNA P-glycoprotein Survivin Multidrug resistance |
| ISSN号 | 0142-9612 |
| DOI | 10.1016/j.biomaterials.2012.05.039 |
| 文献子类 | Article |
| 英文摘要 | Multidrug resistance (MDR) remains one of the main challenges in the successful chemotherapy of human cancer. RNA interference (RNAi) strategy aiming at only one cause of MDR was widely applied, nevertheless hardly obtained satisfactory tumor-suppressing effect. In this work, a new attempt to package two kinds of RNA with different functions into one vector and reverse MDR against two different mechanisms via RNAi was carried out. A new bioreducible poly (beta-amino esters) (PAEs), poly[bis(2-hydroxylethyl)-disulfide-diacrylate-beta-tetraethylenepentamine] (PAP) was synthesized by Michael addition reaction. The PAEs/RNA complex nanoparticles (PAEN) were prepared. The experimental results demonstrated that co-delivery of iMdr-1-shRNA and iSurvivin-shRNA could be achieved by a single vector, and interfering two genes simultaneously had a synergistic effect on overcoming MDR. PAEN lowered the IC50 value of doxorubicin (DOX) in MDR tumor cells to a comparable level to that in the sensitive cell line through down-regulating the expression of P-gp and Survivin, and decreased the tumor volumes in mice xenograft model bearing DOX-resistant human breast cancer when combined with DOX. These results illustrated that PAEN could be applied as potential efficient non-viral RNA carriers for reversing MDR. (c) 2012 Elsevier Ltd. All rights reserved. |
| WOS关键词 | DRUG-RESISTANCE ; GENE-EXPRESSION ; P-GP ; CANCER ; NANOPARTICLES ; CELLS ; DOXORUBICIN ; TRANSPORTERS ; INHIBITION ; VERAPAMIL |
| 资助项目 | The National Basic Research Program of China[2010CB934000] ; The National Basic Research Program of China[2012CB932500] ; National Natural Science Foundation of China[30925041] ; National Natural Science Foundation of China[81102388] ; Shanghai Elitist Program[11XD1406200] |
| WOS研究方向 | Engineering ; Materials Science |
| 语种 | 英语 |
| WOS记录号 | WOS:000307148200020 |
| 出版者 | ELSEVIER SCI LTD |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/277979]  |
| 专题 | 药物制剂研究中心 |
| 通讯作者 | Li, Yaping |
| 作者单位 | Chinese Acad Sci, Shanghai Inst Mat Med, Ctr Pharmaceut, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yin, Qi,Shen, Jianan,Chen, Lingli,et al. Overcoming multidrug resistance by co-delivery of Mdr-1 and survivin-targeting RNA with reduction-responsible cationic poly(beta-amino esters)[J]. BIOMATERIALS,2012,33(27):6495-6506. |
| APA | Yin, Qi,Shen, Jianan,Chen, Lingli,Zhang, Zhiwen,Gu, Wangwen,&Li, Yaping.(2012).Overcoming multidrug resistance by co-delivery of Mdr-1 and survivin-targeting RNA with reduction-responsible cationic poly(beta-amino esters).BIOMATERIALS,33(27),6495-6506. |
| MLA | Yin, Qi,et al."Overcoming multidrug resistance by co-delivery of Mdr-1 and survivin-targeting RNA with reduction-responsible cationic poly(beta-amino esters)".BIOMATERIALS 33.27(2012):6495-6506. |
入库方式： OAI收割
来源：上海药物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。