Low Molecular Weight Polyethylenimine-graft-Tween 85 for Effective Gene Delivery: Synthesis and in Vitro Characteristics
文献类型：期刊论文
| 作者 | Xiao, Jisheng1; Duan, Xiaopin1,2; Yin, Qi1 ; Chen, Lingli1; Zhang, Zhiwen1; Li, Yaping1
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| 刊名 | BIOCONJUGATE CHEMISTRY
 |
| 出版日期 | 2012-02 |
| 卷号 | 23期号:2页码:222-231 |
| ISSN号 | 1043-1802 |
| DOI | 10.1021/bc200504v |
| 文献子类 | Article |
| 英文摘要 | The development of safe and efficient gene delivery systems is still a challenge for successful gene therapy. In this work, low molecular weight polyethylenimine (PEI 2K) was modified by Tween 85, which bears three oleate chains. Tween 85 modified PEI 2K (TP) could condense DNA efficiently, and TP/DNA complexes (TPCs) showed high resistance to salt-induced aggregation and enzymatic degradation. In addition, TP did not show the obvious cytotoxicity. The introduction of Tween 85 led to a significant increase in the cellular uptake of complexes with higher transfection efficiency, which was strongly inhibited by the addition of free Tween 85 in MCF-7/ADR cells, but not in MCF-7 cells. These results indicated that TP could be a potentially safe and effective copolymer for gene delivery, and TPCs could be taken up mainly by Tween 85-mediated endocytosis in MCF-7/ADR cells. |
| WOS关键词 | TRANSFECTION EFFICIENCY ; VECTORS ; DNA ; NANOPARTICLES ; PEI ; DERIVATIVES ; COPOLYMERS ; GLYCOL) ; DESIGN ; LIPIDS |
| 资助项目 | National Basic Research Program of China[2010CB934000] ; National Basic Research Program of China[2012CB932500] ; National Natural Science Foundation of China[30925041] ; National Natural Science Foundation of China[81102388] ; Shanghai Elitist Program[11XD1406200] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000300213400009 |
| 出版者 | AMER CHEMICAL SOC |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/278201]  |
| 专题 | 药物制剂研究中心 |
| 通讯作者 | Li, Yaping |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, Ctr Pharmaceut, Shanghai 201203, Peoples R China; 2.Shenyang Pharmaceut Univ, Sch Pharm, Shenyang 110016, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xiao, Jisheng,Duan, Xiaopin,Yin, Qi,et al. Low Molecular Weight Polyethylenimine-graft-Tween 85 for Effective Gene Delivery: Synthesis and in Vitro Characteristics[J]. BIOCONJUGATE CHEMISTRY,2012,23(2):222-231. |
| APA | Xiao, Jisheng,Duan, Xiaopin,Yin, Qi,Chen, Lingli,Zhang, Zhiwen,&Li, Yaping.(2012).Low Molecular Weight Polyethylenimine-graft-Tween 85 for Effective Gene Delivery: Synthesis and in Vitro Characteristics.BIOCONJUGATE CHEMISTRY,23(2),222-231. |
| MLA | Xiao, Jisheng,et al."Low Molecular Weight Polyethylenimine-graft-Tween 85 for Effective Gene Delivery: Synthesis and in Vitro Characteristics".BIOCONJUGATE CHEMISTRY 23.2(2012):222-231. |
入库方式： OAI收割
来源：上海药物研究所
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